Rapid Construction of Capsid-Modified Adenoviral Vectors Through Bacteriophage λ Red Recombination

Abstract

There are extensive efforts to develop cell-targeting adenoviral vectors for gene therapy wherein endogenous cell-binding ligands are ablated and exogenous ligands are introduced by genetic means. Although current approaches can genetically manipulate the capsid genes of adenoviral vectors, these approaches can be timeconsuming and require multiple steps to produce a modified viral genome. We present here the use of the bacteriophage λ Red recombination system as a valuable tool for the easy and rapid construction of capsidmodified adenoviral genomes.