Retinal transduction by intravitreally administered adeno-associated viral (AAV) vector is previously known to be extremely limited to the neural retina except AAV2 capsid type. Recently, we showed that prior laser photocoagulation enhances retinal transduction of intravitreally administered AAV vectors, including the outer retina and retinal pigment epithelium (RPE). Here, by performing short-pulse laser pretreatment on the mouse retina, we demonstrate RPE cells transduced by three different capsid types of AAV vectors, AAV2, AAV5, and AAV8, using RPE wholemounts. For all capsid types, laser pretreatment effectively induced the transduction of RPE cells in and around the laser site.
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References
1.
LeeSH, ColosiP, LeeH, et al.Laser photocoagulation enhances adeno-associated viral vector transduction of mouse retina. Hum Gene Ther Methods, 2014; 25:83–91.
2.
LeeSH, KimHD, ParkYJ, et al.Time-dependent changes of cell proliferation after laser photocoagulation in mouse chorioretinal tissue. Invest Ophthalmol Vis Sci, 2015; 56:2696–2708.
3.
McCreaPD, GuD. The catenin family at a glance. J Cell Sci, 2010; 123:637–642.
4.
DalkaraD, KolstadKD, CaporaleN, et al.Inner limiting membrane barriers to AAV-mediated retinal transduction from the vitreous. Mol Ther, 2009; 17:2096–2102.
