Rat Uterine-Horn Reanastomosis with Prolonged Catheter Use: An Experimental Study

Abstract

Abstract

Objective:

The goal of this study was to perform microscopic and macroscopic analyses of reanastomosis of the rat uterine horn, with and without prolonged Nelaton catheter use.

Materials and Methods:

Nine rats with bilaterally sectioned and reanastomosed uterine horns were analyzed. The left uterine horn was reanastomosed with prolonged use of a Nelaton catheter (group 1; G1), and the right horn was reanastomosed without catheter use (group 0; G0). After 14 days, the rats were euthanized, and their uterine horns were removed. Microscopic and macroscopic analyses were performed.

Results:

Microscopic analysis revealed a fibrotic presence rate of 44.4% and an absence rate of 55.6% in G0. In G1, a higher fibrotic presence rate of 66.6% was found. The inflammatory infiltrate was considered mild in 11.1%, moderate in 55.6%, and intense in 33.3% of the G1 uterine horns, whereas it was considered mild in 66.6%, moderate in 33.3%, and 0% of the G0 uterine horns (p = 0.029). In G0, mild, moderate, and intense neovascularization was found in 5 rats (55.5%), 3 rats (33.3%), and 1 rat (11.1%), respectively, whereas, in G1, mild, moderate, and intense neovascularization was found in 3 rats (33.3%) each (p = 0.470). Granulomas were present in 7 uterine horns (77.8%) in G0 and in 9 uterine horns (100%) in G1. Unexpected findings were only noted in G0.

Conclusions:

Prolonged use of the Nelaton catheter for bilateral reanastomosis of the rat uterine horn led to an increased inflammatory response and an absence of unexpected findings.