Development of a New Multispecies Protein A-Based ELISA for Serodiagnosis of Hepatitis E Infection with Validation in Domestic Swine

Abstract

Hepatitis E virus (HEV) infects roughly 20 million people worldwide, causing self-limiting acute hepatic disease that can evolve into a chronic course. HEV-3, HEV-4, and HEV-7 genotypes are zoonotic and transmitted to humans by consuming raw or undercooked meat. Here, we developed an indirect ELISA based on the recombinant HEV-3 capsid and performed a seroprevalence study on domestic swine in northeastern Brazil. Our in-house ELISA was initially validated using a subset of 79 sera characterized by concordant results for two distinct commercial ELISA kits. Our ELISA exhibited excellent sensitivity (94%) and specificity (100%), with an area under the curve of 0.99 Further testing, including 212 swine sera, revealed a seroprevalence of 57.5% (95% confidence interval, 50.6–64.3%). Our findings indicate that the novel ELISA test could accurately detect specific anti-HEV antibodies in domestic pigs and should be further validated in humans and other mammals.

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References

Bezerra

, Xavier

, Almeida

, et al. Evaluation of a multi-species Protein A-ELISA assay for plague serologic diagnosis in humans and other mammal hosts. PLoS Negl Trop Dis, 2022; 16(5):e0009805; doi: 10.1371/journal.pntd.0009805

de Oliveira-Filho

, Lopes

, Cunha

, et al. Risk analysis and occurrence of hepatitis E Virus (HEV) in domestic swine in Northeast Brazil. Food Environ Virol, 2017; 9(3):256–259; doi: 10.1007/s12560-017-9292-6

Dos Santos

DRL

, Duraes-Carvalho

, Gardinali

, et al. Uncovering neglected subtypes and zoonotic transmission of Hepatitis E virus (HEV) in Brazil. Virol J, 2023; 20(1):83; doi: 10.1186/s12985-023-02047-6

Gutierrez

, Arce

, Bence

, et al. Unraveling swine hepatitis E in the central region of Argentina through ELISA development and epidemiological insights. Comp Immunol Microbiol Infect Dis, 2023; 103:102082; doi: 10.1016/j.cimid.2023.102082

Moraes

, Mesquita

, Dutra

, et al. Systematic review of hepatitis E virus in Brazil: A one-health approach of the human-animal-environment triad. Animals (Basel), 2021; 11(8); doi: 10.3390/ani11082290

Pandolfi

, Ramos de Almeida

, Alves Pinto

, et al. In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG anti-hepatitis E virus amongst blood donors in south Brazil. PLoS One, 2017; 12(5):e0176409; doi: 10.1371/journal.pone.0176409

Tavares

DHC

, Bezerra

, Magalhaes

, et al. A new recombinant F1 antigen as a cost and time-effective tool for plague diagnosis. J Microbiol Methods, 2020; 172:105903; doi: 10.1016/j.mimet.2020.105903

Wang

, Meng

. Hepatitis E virus: Host tropism and zoonotic infection. Curr Opin Microbiol, 2021; 59:8–15; doi: 10.1016/j.mib.2020.07.004

World Health Organization. Hepatitis E, last update: 20 July 2023. Available from: www.who.int/ [Last accessed: November 13, 2024 ].

10.

Zhao

, Wang

. Laboratory Diagnosis of HEV Infection. Adv Exp Med Biol, 2023; 1417:199–213; doi: 10.1007/978-981-99-1304-6_14

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