Rapid and Specific Methods to Differentiate Foodborne Pathogens,Campylobacter jejuni,Campylobacter coli,and the New Species Causing Spotty Liver Disease in Chickens,Campylobacter hepaticus
Restricted accessResearch articleFirst published online August, 2018
Rapid and Specific Methods to Differentiate Foodborne Pathogens,Campylobacter jejuni,Campylobacter coli,and the New Species Causing Spotty Liver Disease in Chickens,Campylobacter hepaticus
Campylobacter jejuni and Campylobacter coli play a major role in bacteria-related foodborne illness in humans. Recently, a newly identified species, Campylobacter hepaticus, was shown to be the causative agent of spotty liver disease in chickens. The pathogenic potential of C. hepaticus in humans is unknown. This new species contains genes usually used to detect C. jejuni and C. coli in DNA-based detection methods, such as the hippuricase (hipO) gene and the glyA (serine hydroxymethyltransferase) gene, with a high degree of similarity. Therefore, polymerase chain reaction (PCR) primers used to detect these species need to be evaluated carefully to prevent misidentification of these important Campylobacter species. A multiplex PCR was developed and optimized to simultaneously and specifically identify the presence of C. jejuni, C. coli, and C. hepaticus in chicken samples containing high-complexity microbiota. The assay represents a new diagnostic tool for investigating the epidemiology of Campylobacter colonization in poultry and environmental samples. It may also be applicable to the investigation of Campylobacter contamination in food and in outbreaks of campylobacteriosis.
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References
1.
AdziteyF, CorryJ. A comparison between hippurate hydrolysis and multiplex PCR for differentiating Campylobacter coli and Campylobacter jejuni. Trop Life Sci Res, 2011; 22:91–98.
2.
AdziteyF, RusulG, HudaN, CoganT, CorryJ. Prevalence, antibiotic resistance and RAPD typing of Campylobacter species isolated from ducks, their rearing and processing environments in Penang, Malaysia. Int J Food Microbiol, 2012; 154:197–205.
3.
Al AmriA, SenokAC, IsmaeelAY, Al-MahmeedAE, BottaGA. Multiplex PCR for direct identification of Campylobacter spp. in human and chicken stools. J Med Microbiol, 2007; 56:1350–1355.
4.
BurnensDAP, StanleyJ, NicoletJ. Possible association of Helicobacter pullorum with lesions of vibrionic hepatitis in poultry. In: Campylobacters, Helicobacters, and Related Organisms. NewellDG, KetleyJM, FeldmanRA (eds.), Springer US, New York, NY: 1996, pp. 291–293.
5.
CaporasoJG, LauberCL, WaltersWA, Berg-LyonsD, LozuponeCA, TurnbaughPJ, FiererN, KnightR. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. Proc Natl Acad Sci USA, 2011; 108:4516–4522.
6.
CrawshawTR, ChanterJI, YoungSCL, CawthrawS, WhatmoreAM, MarkSK, VidalAB, SalgueroFJ, IrvineRM. Isolation of a novel thermophilic Campylobacter from cases of spotty liver disease in laying hens and experimental reproduction of infection and microscopic pathology. Vet Microbiol, 2015; 179:315–321.
7.
GrimesT, ReeceR. “Spotty liver disease”—An emerging disease in free-range egg layers in Australia. In: Proceedings of the Sixtieth Western Poultry Disease Conference. Presented at the 60th Western Poultry Disease Conference, Sacremento, CA, 2011, pp. 53–56.
8.
JenningsJL, SaitLC, PerrettCA, FosterC, WilliamsLK, HumphreyTJ, CoganTA. Campylobacter jejuni is associated with, but not sufficient to cause vibrionic hepatitis in chickens. Vet Microbiol, 2011; 149:193–199.
9.
JosefsenMH, BhuniaAK, EngvallEO, FachmannMS, HoorfarJ. Monitoring Campylobacter in the poultry production chain—From culture to genes and beyond. J Microbiol Methods, 2015; 112:118–125.
10.
KudirkieneE, MalakauskasM, MalakauskasA, BojesenAM, OlsenJE. Demonstration of persistent strains of Campylobacter jejuni within broiler farms over a 1-year period in Lithuania. J Appl Microbiol, 2010; 108:868–877.
11.
MaddenRH, MoranL, ScatesP, McBrideJ, KellyC. Prevalence of Campylobacter and Salmonella in raw chicken on retail sale in the republic of Ireland. J Food Prot, 2011; 74:1912–1916.
12.
MateoE, CárcamoJ, UrquijoM, PeralesI, Fernández-AstorgaA. Evaluation of a PCR assay for the detection and identification of Campylobacter jejuni and Campylobacter coli in retail poultry products. Res Microbiol, 2005; 156:568–574.
13.
MoffattCR, GreigA, ValcanisM, GaoW, SeemannT, HowdenBP, KirkMD. A large outbreak of Campylobacter jejuni infection in a university college caused by chicken liver pâté, Australia, 2013. Epidemiol Infect, 2016; 144:2971–2978.
14.
NohraA, GrinbergA, MidwinterAC, MarshallJC, Collins-EmersonJM, FrenchNP. Molecular epidemiology of Campylobacter coli strains isolated from different sources in New Zealand between 2005 and 2014. Appl Environ Microbiol, 2016; 82:4363–4370.
PetrovskaL, TangY, Jansen van RensburgMJ, CawthrawS, NunezJ, SheppardSK, EllisRJ, WhatmoreAM, CrawshawTR, IrvineRM. Genome reduction for niche association in Campylobacter hepaticus, ac of Spotty Liver Disease in poultry. Front Cell Infect Microbiol, 2017; 7:354.
17.
SahinO, KassemII, ShenZ, LinJ, RajashekaraG, ZhangQ. Campylobacter in poultry: Ecology and potential interventions. Avian Dis, 2015; 59:185–200.
18.
SzczepanskaB, AndrzejewskaM, SpicaD, and KlaweJJ. Prevalence and antimicrobial resistance of Campylobacter jejuni and Campylobacter coli isolated from children and environmental sources in urban and suburban areas. BMC Microbiol, 2017; 17:80.
19.
TurnerS, PryerKM, MiaoVP, PalmerJD. Investigating deep phylogenetic relationships among cyanobacteria and plastids by small subunit rRNA sequence analysis. J Eukaryot Microbiol, 1999; 46:327–338.
20.
VanTTH, ElshagmaniE, GorMC, ScottPC, MooreRJ. Campylobacter hepaticus sp. nov., isolated from chickens with spotty liver disease. Int J Syst Evol Microbiol, 2016; 66:4518–4524.
21.
VanTTH, ElshagmaniE, GorMC, AnwarA, ScottPC, MooreRJ. Induction of spotty liver disease in layer hens by infection with Campylobacter hepaticus. Vet Microbiol, 2017a;199:85–90.
22.
VanTTH, GorMC, AnwarA, ScottPC, MooreRJ. Campylobacter hepaticus, the cause of spotty liver disease in chickens, is present throughout the small intestine and caeca of infected birds. Vet Microbiol, 2017b;207:226–230.
23.
WangG, ClarkCG, TaylorTM, PucknellC, BartonC, PriceL, WoodwardDL, RodgersFG. Colony Multiplex PCR Assay for identification and differentiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. fetus. J Clin Microbiol, 2002; 40:4744–4747.
24.
Yamazaki-MatsuneW, TaguchiM, SetoK, KawaharaR, KawatsuK, KumedaY, KitazatoM, NukinaM, MisawaN, TsukamotoT. Development of a multiplex PCR assay for identification of Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis. J Med Microbiol, 2007; 56:1467–1473.
