Inhibition of Adhesion of Intestinal Pathogens ( Escherichia coli,Vibrio cholerae,Campylobacter jejuni,and Salmonella Typhimurium) by Common Oligosaccharides

Abstract

Inhibition of the binding of pathogenic adhesins to host glycans by suitable oligosaccharides forms the basis of antiadhesion therapies. Experiments were carried out to study the inhibition capability of oligosaccharides on the adhesion of four microorganisms (Escherichia coli, Vibrio cholerae, Campylobacter jejuni, and Salmonella Typhimurium) to HT-29 cells. Results showed that, in the absence of oligosaccharides, all of the four pathogens efficiently adhered to the cells. Cell adhesion with different bacteria was inhibited by distinct oligosaccharides (e.g., the adhesion number relative to control of V. cholerae could be significantly decreased by pectin oligosaccharide and chitooligosaccharide to about 16.1% and 18.9%, respectively). Saturation studies showed that the extent of antiadhesive effect for most of the suitable carbohydrates was dependent on their concentration. The observations from the study suggest that various carbohydrates may have antiadhesive activity and may be useful in future therapeutic study.