Antimicrobial-resistant bacteria pose a threat to public health. Three Yersinia enterocolitica strains cultured from patients presenting with diarrhea and resistant to nalidixic acid were studied. Target gene mutations in gyrA alone were identified as part of the genetic basis for this phenotype. Efflux activity was also noted, since the presence of the efflux pump inhibitor, phenylalanine-arginine-β-naphthylamide, increased susceptibility to nalidixic acid.
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References
1.
CapillaS, RuizJ, GoñiP, CastilloJ, RubioMC, Jiménez de AntaMT, Gómez-LusR, VilaJ. Characterization of the molecular mechanisms of quinolone resistance in Yersinia enterocolitica O:3 clinical isolates. J Antimicrob Chemother, 2004; 53:1068–1071.
2.
CattoirV, PoirelL, RotimiV, SoussyCJ, NordmannP. Multiplex PCR for detection of plasmid-mediated quinolone resistance qnr genes in ESBL-producing enterobacterial isolates. J Antimicrob Chemother, 2007a. 60:394–397.
3.
CattoirV, WeillFX, PoirelL, FabreL, SoussyCJ, NordmannP. Prevalence of qnr genes in Salmonella in France. J Antimicrob Chemother, 2007b. 59:751–754.
4.
CavacoLM, HasmanH, XiaS, AarestrupFM. qnrD, a novel gene conferring transferable quinolone resistance in Salmonella enterica serovar Kentucky and Bovismorbificans strains of human origin. Antimicrob Agents Chemother, 2009; 53:603–608.
5.
[CLSI] Clinical Laboratory Standards Institute. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard. 3rdCLSI Document, M31–A3. Wayne, PA: CLSI, 2008.
6.
DrummondN, MurphyBP, RingwoodT, PrenticeMB, BuckleyJF, FanningS. Yersinia enterocolitica: A brief review of the issues relating to the zoonotic pathogen, public health challenges and the pork production chain. Foodborne Pathog Dis, 2012; 9:179–189.
7.
FàbregaA, RocaI, VilaJ. Fluoroquinolone and multidrug resistance phenotypes associated with the overexpression of AcrAB and an orthologue of MarA in Yersinia enterocolitica. Int J Med Microbiol, 2010; 300:457–463.
8.
FalcãoJP, BrocchiM, Proenca-ModenaJL, AcraniGO, CorreaEF, FalcãoDP. Virulence characteristics and epidemiology of Yersinia enterocolitica and Yersiniae other than Y. pseudotuberculosis and Y. pestis isolated from water and sewage. J Appl Microbiol, 2004; 96:1230–1236.
9.
Fredriksson-AhomaaM, CernelaN, HächlerH, StephanR. Yersinia enterocolitica strains associated with human infections in Switzerland 2001–2010. Eur J Clin Microbiol Infect Dis, 2012; 31:1543–1550.
10.
KarczmarczykM, MartinsM, QuinnT, LeonardN, FanningS. Mechanisms of fluoroquinolone resistance in Escherichia coli isolates from food-producing animals. Appl Environ Microbiol, 2011; 77:7113–7120.
11.
LantzPG, KnutssonR, BlixtY, Abu Al-SoudW, BorchE, RadostromP. Detection of pathogenic Yersinia enterocolitica in enrichment media and pork by a multiplex PCR: A study of sample preparation and PCR-inhibitory components. Int J Food Microbiol, 1998; 45:93–105.
12.
MurphyBP, DrummondN, RingwoodT, O'SullivanE, BuckleyJF, WhyteP, PrenticeMB, FanningS. First report: Yersinia enterocolitica recovered from canine tonsils in Ireland. Vet Microbiol, 2010; 146:336–339.
13.
ParkCH, RobicsekA, JacobyGA, SahmD, HooperDC. Prevalence in the United States of aac(6')-Ib-cr encoding a ciprofloxacin-modifying enzyme. Antimicrob Agents Chemother, 2006; 50:3953–3955.
14.
RuizJ. Mechanisms of resistance to quinolones: Target alterations, decreased accumulation and DNA gyrase protection. J Antimicrob Chemother, 2003; 51:1109–1117.
15.
Sánchez-CéspedesJ, NaviaMM, MartínezR, OrdenB, MillánR, RuizJ, VilaJ. Clonal dissemination of Yersinia enterocolitica strains with various susceptibilities to nalidixic acid. J Clin Microbiol, 2003; 41:1769–1771.
16.
SihvonenLM, ToivonenS, HaukkaK, KuusiM, SkurnikM, SiitonenA. Multilocus variable tandem-repeat analysis, pulsed-field gel electrophoresis, and antimicrobial susceptibility patterns in discrimination of sporadic and outbreak-related strains of Yersinia enterocolitica. BMC Microbiol, 2011; 11:42e.
17.
Thisted LambertzS, GranathK, Fredriksson-AhomaaM, JohanssonKE, Danielsson-ThamML. Evaluation of a combined culture and PCR method (NMKL-163A) for detection of presumptive pathogenic Yersinia enterocolitica in pork products. J Food Prot, 2006; 70:335–340.
18.
WangM, GuoQ, XuX, WangX, YeX, WuS, HooperDC, WangM. New plasmid-mediated quinolone resistance gene, qnrC, found in a clinical isolate of Proteus mirabilis. Antimicrob Agents Chemother, 2009; 53:1892–1897.
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