A suspension array assay was developed for molecular serotyping of the seven most prevalent Shiga toxin–producing Escherichia coli (STEC) serogroups (O26, O45, O103, O111, O121, O145, and O157). Fluorescence values of 59 STEC were 30 to >270 times greater than the signals of negative controls, demonstrating the method's effectiveness for the molecular serotyping of STEC.
Get full access to this article
View all access options for this article.
References
1.
[CDC] Centers for Disease Control and Prevention, Department of Health and Human Services. Surveillance for foodborne disease outbreaks—United States. 2007. MMWR Morb Mortal Wkly Rep, 2010; 59:973–979.
2.
CebulaTA, PayneWL, FengP. Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR. J Clin Microbiol, 1995; 33:248–250.
3.
DunbarSA. Applications of Luminex xMAP technology for rapid, high-throughput multiplexed nucleic acid detection. Clin Chim Acta, 2006; 363:71–82.
4.
FitzgeraldC, CollinsM, Van DuyneS, MikoleitM, BrownT, FieldsP. Multiplex, bead-based suspension array for molecular determination of common Salmonella serogroups. J Clin Microbiol, 2007; 45:3323–3334.
5.
FratamicoPM, DebroyC, MiyamotoT, LiuY. PCR detection of enterohemorrhagic Escherichia coli O145 in food by targeting genes in the E. coli O145 O-antigen gene cluster and the shiga toxin 1 and shiga toxin 2 genes. Foodborne Pathog Dis, 2009; 6:605–611.
6.
FultonRJ, McdadeRL, SmithPL, KienkerLJ, KettmanJR. Advanced multiplexed analysis with the FlowMetrix system. Clin Chem, 1997; 43:1749–1756.
7.
JohnsonKE, ThorpeCM, SearsCL. The emerging clinical importance of non-O157 Shiga toxin–producing Escherichia coli. Clin Infect Dis, 2006; 43:1587–1595.
8.
LinA, NguyenL, LeeT, ClotildeLM, KaseJA, SonI, CarterJM, LauzonCR. Rapid O serogroup identification of the ten most clinically relevant STECs by Luminex microbead-based suspension array. J Microbiol Methods, 2011; 87:105–110.
9.
PatonAW, PatonJC. Detection and charactertization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorragic E. coli hlyA, rfbO111, and rfbO157. J Clin Microbiol, 1998; 36:598–602.
10.
SamuelG, ReevesP. Biosynthesis of O-antigens: Genes and pathways involved in nucleotide sugar precursor synthesis and O-antigen assembly. Carbohydr Res, 2003; 338:2503–2519.
11.
[USDA] United States Department of Agriculture, Food Safety and Inspection Service. Shiga Toxin–Producing Escherichia coli in Certain Raw Beef Products. 2012. www.fsis.usda.gov/OPPDE/rdad/FRPubs/2010-0023FRN.pdf. 2012May.
