Species Identification of Medically Important Trematodes in Aquatic Food Samples Using PCR-RFLP Targeting 18S rRNA

Abstract

The authors describe a polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method targeting the 18S rRNA gene for the species detection of medically important trematode infecting fish and oysters, and suggest that this PCR-RFLP method based on a specific Tre-18 primer and the restriction enzymes, Acc1, Ava2, Msp1, and Hinf1, is useful for the detection of parasites in aquatic food.