An acetone-sodium dodecyl sulfate disruption method was used for the extraction of cellular proteins from neurotoxigenic Clostridium botulinum. The amount of protein extracted per gram of dry weight and the protein profile as revealed by polyacrylamide gel electrophoresis was comparable to the extracts prepared by established methods, namely, sonication or agitation with beads. This method allows safer handling of C. botulinum by avoiding mechanical disruption, the generation of aerosols, and contamination of the apparatus.
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