Sage Journals: Discover world-class research

Abstract

To examine the serotype specificity of inlH, which encodes the virulence-associated surface protein InlH related to the intracellular survival of Listeria monocytogenes in mice, the presence of inlH in 337 L. monocytogenes strains, representing 11 different serotypes, was studied. A total of 106 strains representing 3 serotypes and 14 pulsed-field gel electrophoresis (PFGE) types were positive for inlH by polymerase chain reaction. inlH was present in all 99 serotype 1/2c and 3 serotype 3c strains. Moreover, 4 out of 129 (3%) serotype 1/2a strains carried inlH. All 106 strains representing serotypes 1/2b, 3a, 3b, 4a, 4b, 4c, 4d, and 7 and 125 out of 129 (97%) serotype 1/2a strains were inlH-negative. The coding sequences of the inlH genes of eight L. monocytogenes strains representing three serotypes and five PFGE types were identical. These results suggest that inlH is specifically present in serotype 1/2c, 3c, and a small fraction of 1/2a L. monocytogenes strains and exists as a single allele.

Get full access to this article

View all access options for this article.

References

Autio

, Lundén

, Fredriksson-Ahomaa

et al. Similar Listeria monocytogenes pulsotypes detected in several foods originating from different sources. Int J Food Microbiol, 2002; 77:83–90.

Broad Institute. www.broadinstitute.org/annotation/genome/listeria_group/MultiHome.html. 2011 March 22. (Online.)

Chen

, Luo

, Jiang

et al. Molecular characteristics and virulence potential of Listeria monocytogenes isolates from Chinese food systems. Food Microbiol, 2009; 26:103–111.

Doumith

, Cazalet

, Simoes

et al. New aspects regarding evolution and virulence of Listeria monocytogenes reveald by comparative genomics and DNA arrays. Infect Immun, 2004; 72:1072–1083.

Dramsi

, Biswas

, Maguin

et al. Entry of Listeria monocytogenes into hepatocytes requires expression of inlB, a surface protein of the internalin multigene family. Mol Microbiol, 1995; 16:251–261.

Gaillard

J-L

, Berche

, Frehel

et al. Entry of L. monocytogenes into cells is mediated by internalin, a repeat protein reminiscent of surface antigens from gram-positive cocci. Cell, 1991; 65:1127–1141.

Glaser

, Frangeul

, Buchrieser

et al. Comparative genomics of Listeria species. Science, 2001; 294:849–852.

Jia

, Nightingale

, Boor

et al. Distribution of internalin gene profiles of Listeria monocytogenes isolates from different sources associated with phylogenetic lineages. Foodborne Pathog Dis, 2007; 4:222–232.

Nelson

, Fouts

, Mongodin

et al. Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species. Nucleic Acids Res, 2004; 32:2386–2395.

10.

Personnic

, Bruck

, Nahori

M-A

et al. The stress-induced protein InlH controls interleukin-6 production during murine listeriosis. Infect Immun, 2010; 78:1979–1989.

11.

Raffelsbauer

, Bubert

, Engelbrecht

et al. The gene cluster inlC2DE of Listeria monocytogenes contains additional new internalin genes and is important for virulence in mice. Mol Gen Genet, 1998; 260:144–158.

12.

Sabet

, Toledo-Arana

, Personnic

et al. The Listeria monocytogenes virulence factor InlJ is specifically expressed in vivo and behaves as an adhesin. Infect Immun, 2008; 76:1368–1378.

13.

Schubert

W-D

, Göbel

, Diepholz

et al. Internalins from the human pathogen Listeria monocytogenes combine three distinct folds into a contiguous internalin domain. J Mol Biol, 2001; 312:783–794.

14.

Tsai

Y-HL

, Orsi

, Nightingale

, Wiedmann

. Listeria monocytogenes internalins are highly diverse and evolved by recombination and positive selection. Infect Genet Evol, 2006; 6:378–389.

15.

Ward

, Gorski

, Borucki

et al. Intraspecific phylogeny and lineage group identification based on the prfA virulence gene cluster of Listeria monocytogenes. J Bacteriol, 2004; 186:4994–5002.

Listeria monocytogenes Serotypes 1/2c and 3c Possess inlH

Abstract

Get full access to this article

References