In this article, the sensitivity of Salmonella enteritidis to surfactin and polylysine was observed, and the optimization of antimicrobial activity of surfactin and polylysine against S. enteritidis in milk was evaluated by a response surface methodology. Results showed that S. enteritidis was very sensitive to them, whose minimal inhibitory concentrations were 6.25 and 31.25 μg/mL, respectively. The optimization result indicated that S. enteritidis could be sterilized by 6 orders of magnitude when the temperature was 4.45°C, the action time was 6.91 h, and the concentration (surfactin/polylysine weight ratio, 1:1) was 10.03 μg/mL.
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References
1.
ChenY, MontvilleTJ. Efflux of ions and ATP depletion induced by pediocin PA-1 are concomitant with cell death in Listeria monocytogenes Scott A. J Appl Microbiol, 1995; 76:684–690.
2.
ChoSJ, LeeSK, ChaBJ, KimYH, ShinKS. Detection and characterization of the Gloeosporium gloeosporioides growth inhibitory compound iturin A from Bacillus subtilis strain KS03. FEMS Microbiol Lett, 2003; 223:47–51.
3.
KajimuraY, SugiyamaM, KanedaM. Bacillopeptins, new cyclic lipopeptide antibiotics from Bacillus subtilis FR-2. J Antibiot (Tokyo), 1995; 48:1095–1103.
4.
KlugeB, VaterJ, SalnikowJ, EckartK. Studies on the biosynthesis of surfactin, a lipopeptide antibiotic from Bacillus subtilis ATCC 21332. FEBS Lett, 1988; 231:107–110.
5.
LiuBL, TzengYM. Optimization of growth medium for the production of spores from Bacillus thuringiensis using response surface methodology. Bioprocess Eng, 1998; 18:413–418.
6.
ReddyPRM, MrudulaS, RameshB, ReddyG, SeenayyaG. Production of thermostable pullulanase by clostridium thermosulfurogenes SV2 in solid-state fermentation: optimization of enzyme leaching conditions using response surface methodology. Bioprocess Eng, 2000; 23:107–112.
7.
ShuHY, LinGH, WuYC, TschenJS, LiuST. Amino acids activated by fengycin synthetase FenE. Biochem Biophys Res Commun, 2002; 292:789–793.
8.
SiragusaGR, CutterCN, WillettJL. Incorporation of bacteriocin in plastic retains activity and inhibits surface growth of bacteria on meat. Food Microbiol, 1999; 16:229–235.
9.
StellerS, VaterJ. Purification of the fengycin synthetase multienzyme system from Bacillus subtilis b213. J Chromatogr B Biomed Sci Appl, 2000; 737:267–275.
10.
TrupkinS, LevinL, ForchiassinF. Optimization of a culture medium for ligninolytic enzyme production and synthetic dye decolorization using response surface methodology. J Ind Microbiol Biotechnol, 2003; 30:682–690.
11.
VorlandLH, UlvatneH, AndersenJ, HauklandH, RekdalO, SvendsenJS, GuttebergTJ. Lactoferricin of bovine origin is more active than lactoferricins of human, murine and caprine origin. Scand J Infect Dis, 1998; 30:513–517.
