Phenotypic and Genotypic Traits of Shiga Toxin–Negative E. coli O157:H7/H − Bovine and Porcine Strains

Abstract

Enterohemorrhagic E. coli (EHEC) O157:H7/H⁻ (nonmotile) exist as Shiga toxin gene (stx)-positive and stx-negative variants in patients and the environment. We analyzed molecular characteristics, phenotypes, and the phylogenetic background of three stx-negative E. coli O157:H7/H⁻ strains isolated from cattle and a pig and compared them with those of human EHEC and stx-negative E. coli O157:H7/H⁻. All three animal strains contained fliCH7 and two contained eae. One eae-positive strain (O157:H⁻) was sorbitol-fermenting (SF) and the other (O157:H7) was non–sorbitol-fermenting (nSF). These two strains shared a spectrum of non-stx putative virulence and fitness genes with human nSF and SF EHEC and stx-negative E. coli O157:H7/H⁻ and belonged, similar to the vast majority of human isolates, to sequence type (ST) 11 in multilocus sequence typing. In contrast, the eae-negative O157:H7 animal isolate, which was SF, differed in spectrum of virulence genes and also differed phylogenetically (ST717) from the two eae-positive strains and the human EHEC and stx-negative E. coli O157:H7/H⁻. In contrast to efforts with human stx-negative E. coli O157:H⁻, attempts to transduce the two stx-negative/eae-positive animal O157:H7/H⁻ strains with stx ₂-encoding phages from human SF and nSF EHEC O157:H7/H⁻ failed, despite the animal strains having intact loci where such phages integrate in human EHEC O157 (wrbA and yecE). The role of animal stx-negative/eae-positive and stx-negative/eae-negative E. coli O157:H7/H⁻ in their natural source and in human infections needs further investigation.