Study of Clostridium botulinum by Various Formulations of Salt and Preservatives in Persian Caviar

Abstract

Abstract

This article reports on growth and toxigenesis of Clostridium botulinum type E beluga in Persian granular. Sixty-three formulations of NaCl and 3 additives were examined in different conditions in Acipenser persicus caviar. The primary inoculated spores were 5.1 × 10⁴ colony-forming units (CFU)/g of caviar. Count of spores in mixture of sodium chloride (NaCl) plus 0.3% boric acid and 0.4 % borax treatments were altered to 1.56 × 10⁴, 3.65 × 10⁴ and 9.22 × 10⁴ CFU/g, respectively. Toxin production was positive in 5% NaCl, NaCl plus 0.3% boric acid and 0.4% borax after 14 days. Toxigenesis was negative at −2 degrees in all treatments, 5°C in B and C treatments, and 15°C in mixture of NaCl plus 0.15 % methyl paraben treatments. Methyl paraben have had more inhibitory effect than boric acid, borax, and NaCl. Because of restricted use of boric acid and borax in granular caviar in many countries, methyl paraben, which is a GRAS (generally recognized as safe) preservative and has a strong antimicrobial effect at the pH of caviar, is recommended.