Constructed wetland-microbial fuel cell (CW-MFC) has excellent effect and potential in wastewater treatment based on many factors, such as fillers, hydraulic retention time (HRT), influent chemical oxygen demand (COD) concentration, and plants. This study explored the influence of fillers, influent COD concentrations, and HRT on the performance of CW-MFC systems to optimize the operation. Two CW-MFCs with pyrite and activated carbon as anode materials (PCW-MFC and ACW-MFC) were set up, respectively. The results showed that prolonging HRT of the systems could improve the nutrients’ removal efficiency and voltage output. The influent COD concentration had a positive import on the removal efficiency of COD, nitrate-nitrogen (NO3−-N), and total phosphorus (TP) in CW-MFCs. On the contrary, the removal efficiency of ammonia-nitrogen (NH4+-N) decreased and the voltage change showed a trend of increasing first and then decreasing with the increase of COD concentration. The optimal operating conditions of CW-MFCs were COD of 200 mg/L and HRT of 24 h. Moreover, it found that the overall performance of PCW-MFC was better than that of ACW-MFC. Microbial community analysis evaluated that the abundance of autotrophic denitrifying bacteria Thiobacillus, Dechloromonas, and Pseudomonas in the cathode layer of PCW-MFC was higher than that of ACW-MFC, indicating that pyrite could effectively promote the bacterial reproduction of nitrogen and phosphorus removal and electrogenesis.
CaiQ, LaiC, YangP. Biomass yield characteristics and removal kinetic model construction with mass transfer optimization of a Moving Bed and Constructed Wetland (MBCW) integrated bioreactor. Biochem Eng J, 2023; 200:109085; doi: 10.1016/j.bej.2023.109085
3.
ChevalierS, BouffartiguesE, BodilisJ, et al.Structure, function and regulation of Pseudomonas Aeruginosa Porins. FEMS Microbiol Rev, 2017; 41(5):698–722; doi: 10.1093/femsre/fux020
4.
de-BashanLE, BashanY. Recent advances in removing phosphorus from wastewater and its future use as fertilizer (1997–2003). Water Res, 2004; 38(19):4222–4246; doi: 10.1016/j.watres.2004.07.014
5.
DohertyL, ZhaoY, ZhaoX, et al.Nutrient and organics removal from swine slurry with simultaneous electricity generation in an alum sludge-based constructed wetland incorporating microbial fuel cell technology. Chem Eng J, 2015; 266:74–81; doi: 10.1016/j.cej.2014.12.063
6.
EbrahimiA, SivakumarM, McLauchlanC, et al.A critical review of the symbiotic relationship between constructed wetland and microbial fuel cell for enhancing pollutant removal and energy generation. J Environ Chem Eng, 2021; 9(1):105011; doi: 10.1016/j.jece.2020.105011
7.
FanT, WangM, WangX, et al.Experimental study of the adsorption of nitrogen and phosphorus by natural clay minerals. In: Adsorpt Sci Technol. (DuH ed.) Sage; 2021; 2021:1–14; doi: 10.1155/2021/4158151
8.
FaulwetterJL, GagnonV, SundbergC, et al.Microbial processes influencing performance of treatment wetlands: A review. Ecol Eng, 2009; 35(6):987–1004; doi: 10.1016/j.ecoleng.2008.12.030
9.
Fdz-PolancoF, Fdz-PolancoM, FernandezN, et al.New process for simultaneous removal of nitrogen and sulphur under anaerobic conditions. Water Res, 2001; 35(4):1111–1114; doi: 10.1016/S0043-1354(00)00474-7
10.
FengY, HeW, LiuJ, et al.A horizontal plug flow and stackable pilot microbial fuel cell for municipal wastewater treatment. Bioresour Technol, 2014; 156:132–138; doi: 10.1016/j.biortech.2013.12.104
11.
FuG, WuJ, HanJ, et al.Effects of substrate type on denitrification efficiency and microbial community structure in constructed wetlands. Bioresour Technol, 2020; 307:123222; doi: 10.1016/j.biortech.2020.123222
12.
GeZ, WeiD, ZhangJ, et al.Natural pyrite to enhance simultaneous long-term nitrogen and phosphorus removal in constructed wetland: Three years of pilot study. Water Res, 2019; 148:153–161; doi: 10.1016/j.watres.2018.10.037
13.
HongX, ChenZ, ZhaoC, et al.Nitrogen transformation under different dissolved oxygen levels by the anoxygenic phototrophic bacterium marichromatium gracile. World J Microbiol Biotechnol, 2017; 33(6):113; doi: 10.1007/s11274-017-2280-z
14.
HuangX, DuanC, DuanW, et al.Role of electrode materials on performance and microbial characteristics in the Constructed Wetland Coupled Microbial Fuel Cell (CW-MFC): A review. J Clean Prod, 2021; 301:126951; doi: 10.1016/j.jclepro.2021.126951
15.
HuoJ, HuX, ChengS, et al.Effects and mechanisms of constructed wetlands with different substrates on N2O emission in wastewater treatment. Environ Sci Pollut Res Int, 2022; 29(13):19045–19053; doi: 10.1007/s11356-021-17219-6
16.
HussainSI, BlowesDW, PtacekCJ, et al.Mechanisms of phosphorus removal in a pilot-scale constructed wetland/BOF slag wastewater treatment system. Environ Eng Sci, 2015; 32(4):340–352; doi: 10.1089/ees.2014.0376
17.
Juncher JørgensenC, JacobsenOS, ElberlingB, et al.Microbial oxidation of pyrite coupled to nitrate reduction in anoxic groundwater sediment. Environ Sci Technol, 2009; 43(13):4851–4857; doi: 10.1021/es803417s
18.
KhanthongK, JangH, KadamR, et al.Bioelectrochemical system for nitrogen removal: Fundamentals, current status, trends, and challenges. Chemosphere, 2023; 339:139776; doi: 10.1016/j.chemosphere.2023.139776
19.
KongY, TaoM, LuX, et al.Study on the performance of accessory microbial fuel cell (mfc) integrated to the Constructed Wetlands (CWs) for deep wastewater treatment. Desalination Water Treat, 2024; 318:100358; doi: 10.1016/j.dwt.2024.100358
20.
LiangX, ZhaoH, HeY, et al.Spatiotemporal characteristics of agricultural nitrogen and phosphorus emissions to water and its source identification: A case in Bamen Bay, China. J Contam Hydrol, 2022; 245:103936; doi: 10.1016/j.jconhyd.2021.103936
21.
LimEY, TianH, ChenY, et al.Methanogenic pathway and microbial succession during start-up and stabilization of thermophilic food waste anaerobic digestion with biochar. Bioresour Technol, 2020; 314:123751; doi: 10.1016/j.biortech.2020.123751
22.
LiuY, LiuX, WangH, et al.Pyrite coupled with steel slag to enhance simultaneous nitrogen and phosphorus removal in constructed wetlands. Chem Eng J, 2023; 470:143944; doi: 10.1016/j.cej.2023.143944
23.
LiuS, SongH, WeiS, et al.Bio-Cathode materials evaluation and configuration optimization for power output of vertical subsurface flow constructed wetland—Microbial fuel cell systems. Bioresour Technol, 2014; 166:575–583; doi: 10.1016/j.biortech.2014.05.104
24.
LiuX, ZhangY, LiX, et al.Effects of influent nitrogen loads on nitrogen and COD removal in horizontal subsurface flow constructed wetlands during different growth periods of phragmites Australis. Sci Total Environ, 2018; 635:1360–1366; doi: 10.1016/j.scitotenv.2018.03.260
25.
MaY, DaiW, ZhengP, et al.Iron scraps enhance simultaneous nitrogen and phosphorus removal in subsurface flow constructed wetlands. J Hazard Mater, 2020; 395:122612; doi: 10.1016/j.jhazmat.2020.122612
26.
OliveiraCM, MachadoCM, DuarteGW, et al.Beneficiation of pyrite from coal mining. J Clean Prod, 2016; 139:821–827; doi: 10.1016/j.jclepro.2016.08.124
27.
PadhySR, BhattacharyyaP, NayakSK, et al.A unique bacterial and archaeal diversity make mangrove a green production system compared to rice in wetland ecology: A metagenomic approach. Sci Total Environ, 2021; 781:146713; doi: 10.1016/j.scitotenv.2021.146713
28.
SabaB, KhanM, ChristyAD, et al.Microbial phyto-power systems—A sustainable integration of phytoremediation and microbial fuel cells. Bioelectrochemistry, 2019; 127:1–11; doi: 10.1016/j.bioelechem.2018.12.005
29.
SaeedT, SunG. A review on nitrogen and organics removal mechanisms in subsurface flow constructed wetlands: Dependency on environmental parameters, operating conditions and supporting media. J Environ Manage, 2012; 112:429–448; doi: 10.1016/j.jenvman.2012.08.011
30.
SazÇ, TüreC, TürkerOC, et al.Effect of vegetation type on treatment performance and bioelectric production of Constructed Wetland Modules Combined with Microbial Fuel Cell (CW-MFC) treating synthetic wastewater. Environ Sci Pollut Res Int, 2018; 25(9):8777–8792; doi: 10.1007/s11356-018-1208-y
31.
SharmaY, LiB. Optimizing energy harvest in wastewater treatment by combining anaerobic Hydrogen Producing Biofermentor (HPB) and Microbial Fuel Cell (MFC). Int J Hydrog Energy, 2010; 35(8):3789–3797; doi: 10.1016/j.ijhydene.2010.01.042
32.
ShenX, ZhangJ, LiuD, et al.Enhance performance of microbial fuel cell coupled surface flow constructed wetland by using submerged plants and enclosed anodes. Chem Eng J, 2018; 351:312–318; doi: 10.1016/j.cej.2018.06.117
33.
TaoM, KongY, CaoS, et al.Constructed Wetland—Microbial Fuel Cell (CW-MFC) packed with suspended fillers to enhance denitrification with acorus calamus biomass addition. Chem Eng J, 2024; 487:150753; doi: 10.1016/j.cej.2024.150753
34.
TaoM, KongY, JingZ, et al.Denitrification performance, bioelectricity generation and microbial response in microbial fuel cell—Constructed wetland treating carbon constraint wastewater. Bioresour Technol, 2022; 363:127902; doi: 10.1016/j.biortech.2022.127902
35.
VelvizhiG, Venkata MohanS. Electrogenic activity and electron losses under increasing organic load of recalcitrant pharmaceutical wastewater. Int J Hydrog Energy, 2012; 37(7):5969–5978; doi: 10.1016/j.ijhydene.2011.12.112
36.
WangT, LinZ, KuangB, et al.Electroactive algae-bacteria wetlands for the treatment of micro-polluted aquaculture wastewater: Pilot-Scale verification. Biochem Eng J, 2022; 184:108471; doi: 10.1016/j.bej.2022.108471
37.
WangJ, SongX, WangY, et al.Bioenergy generation and rhizodegradation as affected by microbial community distribution in a coupled constructed wetland-microbial fuel cell system associated with three macrophytes. Sci Total Environ, 2017; 607–608:53–62; doi: 10.1016/j.scitotenv.2017.06.243
38.
WangX, TianY, LiuH, et al.Effects of influent COD/TN ratio on nitrogen removal in integrated constructed wetland–microbial fuel cell systems. Bioresour Technol, 2019; 271:492–495; doi: 10.1016/j.biortech.2018.09.039
39.
WeiL, WangG, JiangJ, et al.Co-Removal of phosphorus and nitrogen with commercial 201 × 7 anion exchange resin during tertiary treatment of WWTP effluent and phosphate recovery. Desalination Water Treat, 2015; 56(6):1633–1647; doi: 10.1080/19443994.2014.951966
40.
XieT, JingZ, HuJ, et al.Degradation of Nitrobenzene-Containing wastewater by a microbial-fuel-cell-coupled constructed wetland. Ecol Eng, 2018; 112:65–71; doi: 10.1016/j.ecoleng.2017.12.018
41.
XuF, SunR, WangH, et al.Improving the outcomes from electroactive constructed wetlands by mixing wastewaters from different beverage-processing industries. Chemosphere, 2021; 283:131203; doi: 10.1016/j.chemosphere.2021.131203
42.
YadavAK, DashP, MohantyA, et al.Performance assessment of innovative constructed wetland-microbial fuel cell for electricity production and dye removal. Ecol Eng, 2012; 47:126–131; doi: 10.1016/j.ecoleng.2012.06.029
43.
YangH, ChenJ, YuL, et al.Performance optimization and microbial community evaluation for domestic wastewater treatment in a constructed wetland-microbial fuel cell. Environ Res, 2022; 212(Pt B):113249; doi: 10.1016/j.envres.2022.113249
44.
YangZ, ZhouQ, SunH, et al.Metagenomic analyses of microbial structure and metabolic pathway in solid-phase denitrification systems for advanced nitrogen removal of wastewater treatment plant effluent: A pilot-scale study. Water Res, 2021; 196:117067; doi: 10.1016/j.watres.2021.117067
45.
ZagklisDP, BamposG. Tertiary wastewater treatment technologies: A review of technical, economic, and life cycle aspects. Processes, 2022; 10(11):2304; doi: 10.3390/pr10112304
46.
ZhangD, HanW, YanC, et al.Treatment of swine wastewater using multi-soil-layer based constructed wetland: Substrates assessment and efficiency improvement. Biochem Eng J, 2022; 188:108679; doi: 10.1016/j.bej.2022.108679
47.
ZhangL, LiuY, LvS, et al.An overview on constructed wetland-microbial fuel cell: Greenhouse gases emissions and extracellular electron transfer. J Environ Chem Eng, 2023; 11(2):109551; doi: 10.1016/j.jece.2023.109551
48.
ZhaoL, XueN, LuZ, et al.Pyrrhotite-Based constructed wetland–microbial fuel cell: Reactive Brilliant Red X-3B removal performance and microbial communities. J Environ Eng, 2023; 149(1):4022086; doi: 10.1061/JOEEDU.EEENG-6958
49.
ZhuH, YanB, XuY, et al.Removal of nitrogen and COD in horizontal subsurface flow constructed wetlands under different influent C/N ratios. Ecol Eng, 2014; 63:58–63; doi: 10.1016/j.ecoleng.2013.12.018
