The plants being sessile cannot escape from the adverse environmental stresses, hence get negatively affected in terms of their growth and yield. Transcriptional control simultaneously regulate different cellular processes, minimizing the deleterious effects of these stresses. The salicylic acid (SA)-inducible WRKY family of transcription factors auto or crossregulate the stress signaling in response to abiotic and biotic stresses, facilitating enhanced stress tolerance. In this study, we characterized the group III WRKY gene, JcWRKY2 from ecological and economical valued shrub Jatropha curcas. The JcWRKY2 tobacco transgenics showed improved physiological growth parameters, elevated chlorophyll content, improved antioxidative activities, and increased endogenous SA with both salt and SA stress. Interestingly, the pretreatment with SA and hydrogen peroxide facilitated improved germination of transgenic seeds with salinity stress. The transgenics showed differential regulation of antioxidative enzymes, calcium/calmodulin, dehydrins, and phospholipase genes with salt and SA stress. The increased SA content in transgenics on stress treatments, enhanced the antioxidant capacity leading to reduced susceptibility to stresses. Thus, JcWRKY2 transgenics participate in SA-mediated, improved antioxidative status during salinity stress with reduced reactive oxygen species damage.
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