Glycogen synthase kinase-3β (GSK3β) plays a fundamental role in the inflammatory response by controlling the balance between pro- and anti-inflammatory cytokine production induced by lipopolysaccharide (LPS). However, the inflammatory roles of different porcine GSK3β isoforms in response to LPS remain unknown. In this study, the activity of porcine GSK3β was inhibited in response to LPS stimulation through increased serine 9 phosphorylation of GSK3β, which increased the production of interleukin (IL)-10 and IL-12 in PK-15 cells. However, treatment with an inhibitor of GSK3β, lithium chloride, significantly increased the production of IL-10 and IL-12 compared with the untreated control and significantly decreased the production of IL-10 and IL-12 compared with LPS stimulation. Moreover, all four glycogen synthase kinase-3 transcripts had positive roles in the production of IL-10 and IL-12 to varying degrees, suggesting their importance in the LPS-induced inflammatory response in pigs.
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