My 45 years of studying the molecular biology of adeno-associated virus are recounted. Additional activities as a mentor, department chair, and medical school administrator are described, as are my activities in the public sphere, which involved national issues related to science policy and medical education.
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References
1.
BeatonA., PalumboP., BernsK.I.1989. Expression from the adeno-associated virus p5 and p19 promoters is negatively regulated in trans by the rep protein. J Virol, 63:4450–4454.
2.
BernsK.I., SilvermanC.1970. The natural occurrence of cross-linked vaccinia virus DNA. J Virol, 5:299–304.
3.
BernsK.I., ThomasC.A.Jr.1961. A study of single polynucleotide chains derived from T2 and T4 bacteriophage. J Mol Biol, 3:289–300.
4.
BernsK.I., ThomasC.A.Jr.1965. Isolation of high molecular weight DNA from Hemophilus influenza. J Mol Biol, 11:476–490.
5.
BernsK.I., SilvermanC., WeissbachA.1969. Separation of a new deoxyribonucleic acid polymerase from vaccinia-infected HeLa cells. J Virol, 4:15–23.
6.
BohenzkyR.A., LeFebvreR.B., BernsK.I.1988. Sequence and symmetry requirements within the internal palindromic sequences of the adeno-associated virus terminal repeat. Virology, 166:316–327.
7.
CheungA.K.M., HogganM.D., HauswirthW.W., BernsK.I.1980. Integration of the adeno-associated virus genome in cellular DNA in latently infected human Detroit 6 cells. J Virol, 33:739–748.
8.
DayaS., BernsK.I.2009. Adeno-associated virus site-specific integration is mediated by proteins of the nonhomologous end-joining pathway. J Virol, 83:11655–11664.
9.
DyallJ., SzaboP., BernsK.I.1999. Adeno-associated virus (AAV) site-specific integration: formation of AAV-AAVS1 junctions in an in vitro system. Proc Natl Acad Sci U S A, 96:12849–12854.
10.
FifeK.H., BernsK.I., MurrayK.1977. Structure and nucleotide sequence of the terminal regions of adeno-associated virus DNA. Virology, 78:475–487.
11.
GeshelinP., BernsK.I.1974. The characterization of the naturally occurring cross-links in vaccinia virus DNA. J Mol Biol, 88:785–796.
12.
GiraudC., WinocourE., BernsK.I.1994. Site-specific integration by adeno-associated virus is directed by a cellular DNA sequence. Proc Natl Acad Sci U S A, 91:10039–10043.
13.
GrossmanZ., WinocourE., BernsK.I.1984. Recombination between Simian Virus 40 and adeno-associated virus: Virion coinfection compared to DNA cotransfection. Virology, 134:125–137.
14.
HauswirthW.W., BernsK.I.1977. Origin and termination of adeno-associated virus DNA replication. Virology, 78:488–499.
15.
HongG., WardP., BernsK.I.1992. In vitro replication of adeno-associated virus DNA. Proc Natl Acad Sci U S A, 89:4673–4677.
16.
KotinR.M., MenningerJ.C., WardD.C., BernsK.I.1991. Mapping and direct visualization of a region-specific viral DNA integration site on chromosome 19q13-qter. Genomics, 10:831–834.
17.
LabowM., HermonatP.W., BernsK.I.1986. Positive and negative autoregulation of the adeno-associated virus Type 2 genome. J Virol, 60:251–258.
18.
LefebvreR., RivaS., BernsK.I.1984. Conformation takes precedence over sequence in adeno-associated virus DNA replication. Mol Cell Biol, 4:1416–1419.
19.
LeonardC.J., BernsK.I.1994. Cloning, expression, and partial purification of Rep 78: an adeno-associated virus replication protein. Virology, 200:566–573.
20.
LindenR.M., WardP., GiraudC.et al.1996a. Site specific integration by adeno-associated virus. Proc Natl Acad Sci USA, 93:11288–11294.
21.
LindenR.M., WinocourE., BernsK.I.1996b. The recombination signals for adeno-associated virus site-specific integration. Proc Natl Acad Sci U S A, 93:7966–7972.
22.
LusbyE.W., BernsK.I.1982. Mapping of the 5’ termini of two adeno-associated virus2 RNAs in the left half of the genome. J Virol, 41:518–526.
23.
LusbyE., FifeK.H., BernsK.I.1980. The nucleotide sequence of the inverted terminal repetition in adeno-associated virus DNA. J Virol, 34:402–409.
24.
MarmurJ., AndersonW.F., MatthewsL.et al.1961. The effects of ultraviolet light on the biological and physical chemical properties of deoxyribonucleic acids. J Cell Comp Physiol, 58:33–55Suppl, I.
25.
MenesesP.I., HajjarK.A., BernsK.I., DuvoisinR.M.2001. Recombinant angiostatin prevents retinal neovascularization in a murine proliferative retinopathy model. Gene Ther, 8:646–648.
26.
OstroveJ.M., DuckworthD.H., BernsK.I.1981. Inhibition of adenovirus transformed cell oncogenicity by adeno-associated virus. Virology, 113:521–553.
27.
RoseJ.A., BernsK.I., HogganM.D., KoczotF.J.1969. Evidence for a single-stranded adenovirus-associated virus genome: Formation of a DNA density hybrid on release of viral DNA. Proc Nat Acad Sci U S A, 64:863–869.
28.
SamulskiR.J., BernsK.I., TanM., MuzyczkaN.1982. Cloning of AAV into pBR322: Rescue of intact virus from the recombinant plasmid in human cells. Proc Natl Acad Sci U S A, 79:2077–2081.
29.
SpearI., FifeK.H., HauswirthW.W.et al.1977. Evidence for two nucleotide sequence orientations within the terminal repetition of adeno-associated virus DNA. J Virol, 24:627–634.
30.
SrivastavaA., LusbyE.W., BernsK.I.1983. Nucleotide sequence and organization of the adeno-associated virus 2 genome. J Virol, 45:555–556.
31.
ThomasC.A.Jr., BernsK.I.1961. The physical characterization of DNA molecules released from T2 and T4 bacteriophage. J Mol Biol, 3:277–288.
32.
WardP., BernsK.I.1996. In vitro replication of adeno-associated virus DNA: enhancement by extracts from adenovirus-infected HeLa cells. J Virol, 70:4495–4501.
33.
WardP., UrcelayE., KotinR.et al.1994. Adeno-associated virus DNA replication in vitro: activation by a maltose binding protein-rep 68 fusion protein. J Virol, 68:6029–6037.