Restricted accessResearch articleFirst published online 2012-05
Study of DNA-Deltamethrin Binding by Voltammetry,Competitive Fluorescence,Thermal Denaturation,Circular Dichroism,and Atomic Force Microscopy Techniques
The interaction of deltamethrin (DM), a synthetic insecticide, with calf thymus DNA was studied. The cyclic voltammetric (CV) results revealed that DM has two irreversible cathodic peaks. The first peak (a) was devoted to reduction of −CN by 4 electrons and the second peak (b) was devoted to reduction of the −C=C− moiety by two electrons. By using non-linear regression analysis of CV data of peak (a), the binding constant, binding site size, and diffusion coefficient for free DM (Df) and DNA-DM (Db) were calculated as: 2.6×104, 1.6, 3.2×10−4Cm2 S−1, and 8.5×10−6Cm2 S−1, respectively. The thermal denaturation, competitive fluorescence, and AFM results revealed that the mode of interaction may be non-intercalative. Also the circular dichroism spectra showed that the conformation of CT DNA was converted from right-handed B-DNA to A-DNA due to the destacking of the adjacent guanine bases in pH 7.3 solution.
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