Simultaneous Synthesis of Multiple Oligonucleotides Using Nucleoside-H-Phosphonate Intermediates

Abstract

A semimechanized simultaneous synthesis of multiple oligonucleotides using nucleoside-H-phosphonates as intermediates was developed and applied to the preparation of hybridization probes. The synthesis could be done with equivalent results (about 98% average yield per cycle) using regenerated H-phosphonates.