Abstract
The use of homologous recombination in Escherichia coli is described as a tool for DNA manipulation. The utility of the method is illustrated by the addition of 3′-flanking sequences to a dhfr minigene by plasmid-phage recombination involving a supF-containing dhfr minigene plasmid and a λCharon4A phage containing the 3′ end of the dhfr gene. In addition, other uses of both plasmid-phage and phage-phage recombination in gene manipulation are described.
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