Inhibition of Gene Expression at the Translational Level by Cotransfection with Competitor Plasmids

Abstract

We have investigated the effect of cotransfection with other recombinant plasmids on the expression of a test plasmid, p2A9PrCAT, in which the calcyclin promoter directs the expression of the bacterial chloramphenicol transferase (CAT) coding sequence. The expression of CAT is markedly inhibited when p2A9PrCAT is cotransfected with any of several plasmids in which a promoter controls the expression of complete or partial transcribed sequences. A series of experiments indicates that the inhibition is not sequence specific, does not require a full-length protein product, but it requires a transcript, since cotransfection with a vector containing only a promoter gives only a modest inhibition. Although CAT activity is decreased markedly in these competition experiments, the amount of CAT mRNA is unaffected, indicating that the inhibition must take place at a translational level.