CRISPR Adventures in China

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Liu L , Li X , Wang J , et al. Two distant catalytic sites are responsible for C2c2 RNase activities. Cell, 2017; 168:121–134. DOI: 10.1016/j.cell.2016.12.031.

Liu L , Chen P , Wang M , et al. C2c1-sgRNA complex structure reveals RNA-guided DNA cleavage mechanism. Mol Cell, 2017; 65:310–322. DOI: 10.1016/j.molcel.2016.11.040.

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Komor AC , Kim YB , Packer MS , et al. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature, 2016; 533:420–424. DOI: 10.1038/nature17946.

Anzalone AV , Randolph PB , Davis JR , et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature, 2019; 576:149–157. DOI: 10.1038/s41586-019-1711-4.

Li X , Wang Y , Liu Y , et al. Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol, 2018; 36:324–327. DOI: 10.1038/nbt.4102.

Wang X , Li J , Wang Y , et al. Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol, 2018; 36:946–949. DOI: 10.1038/nbt.4198.

Zong Y , Song Q , Li C , et al. Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. Nat Biotechnol, 2018; 36:950–953. DOI: 10.1038/nbt.4261.

Li C , Zhang R , Meng X , et al. Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors. Nat Biotechnol, 2020; 38:875–882. DOI: 10.1038/s41587-019-0393-7.

Zhang X , Zhu B , Chen L , et al. Dual base editor catalyzes both cytosine and adenine base conversions in human cells. Nat Biotechnol, 2020; 38:856–860. DOI: 10.1038/s41587-020-0527-y.

Lin Q , Jin S , Zong Y , et al. High-efficiency prime editing with optimized, paired pegRNAs in plants. Nat Biotechnol 2021 Mar 25 [Epub ahead of print]; DOI: 10.1038/s41587-021-00868-w.

Jin S , Zong Y , Gao Q , et al. Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice. Science, 2019; 364:292–295. DOI: 10.1126/science.aaw7166.

Zuo E , Sun Y , Wei W , et al. Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos. Science, 2019; 364:289–292. DOI: 10.1126/science.aav9973.

Zuo E , Sun Y , Yuan T , et al. A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects. Nat Methods, 2020; 17:600–604. DOI: 10.1038/s41592-020-0832-x.

Jin S , Fei H , Zhu Z , et al. Rationally designed APOBEC3B cytosine base editors with improved specificity. Mol Cell, 2020; 79:728–740.e6. DOI: 10.1016/j.molcel.2020.07.005.

Wang L , Xue W , Zhang H , et al. Eliminating base-editor-induced genome-wide and transcriptome-wide off-target mutations. Nat Cell Biol, 2021; 23:552–563. DOI: 10.1038/s41556-021-00671-4.

Jin S , Lin Q , Luo Y , et al. Genome-wide specificity of prime editors in plants. Nat Biotechnol 2021 Apr 15 [Epub ahead of print]; DOI: 10.1038/s41587-021-00891-x.

Shan Q , Wang Y , Li J , et al. Targeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol, 2013; 31:686–688. DOI: 10.1038/nbt.2650.

Zhu H , Li C , Gao C . Applications of CRISPR-Cas in agriculture and plant biotechnology. Nat Rev Mol Cell Biol, 2020; 21:661–677. DOI: 10.1038/s41580-020-00288-9.

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Lu Y , Ye X , Guo R , et al. Genome-wide targeted mutagenesis in rice using the CRISPR/Cas9 system. Mol Plant, 2017; 10:1242–1245. DOI: 10.1016/j.molp.2017.06.007.

Zhang H , Si X , Ji X , et al. Genome editing of upstream open reading frames enables translational control in plants. Nat Biotechnol, 2018; 36:894–898. DOI: 10.1038/nbt.4202.

Wang Y , Cheng X , Shan Q , et al. Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. Nat Biotechnol, 2014; 32:947–951. DOI: 10.1038/nbt.2969.

Wang C , Liu Q , Shen Y , et al. Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes. Nat Biotechnol, 2019; 37:283–286. DOI: 10.1038/s41587-018-0003-0.

Zhang Y , Liang Z , Zong Y , et al. Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA. Nat Commun, 2016; 7:12617. DOI: 10.1038/ncomms12617.

Liang Z , Chen K , Li T , et al. Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes. Nat Commun, 2017; 8:14261. DOI: 10.1038/ncomms14261.

Yan S , Tu Z , Liu Z , et al. A huntingtin knockin pig model recapitulates features of selective neurodegeneration in Huntington's disease. Cell, 2018; 173:989–1002. DOI: 10.1016/j.cell.2018.03.005.

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Zhou Y , Sharma J , Ke Q , et al. Atypical behaviour and connectivity in SHANK3-mutant macaques. Nature, 2019; 570:326–331. DOI: 10.1038/s41586-019-1278-0.

Niu D , Wei HJ , Lin L , et al. Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9. Science, 2017; 357:1303–1307. DOI: 10.1126/science.aan4187.

Xu L , Wang J , Liu Y , et al. CRISPR-edited stem cells in a patient with HIV and acute lymphocytic leukemia. N Engl J Med, 2019; 381:1240–1247. DOI: 10.1056/NEJMoa1817426.