JinekM, et al.A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science, 2012; 337:816–21.DOI:10.1126/science.1225829.
2.
HsuP, LanderES, ZhangF. Development and applications of CRISPR-Cas9 for genome engineering. Cell, 2014; 157:1262–1278. DOI:10.1016/j.cell.2014.05.010.
3.
HendelA, et al.Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Nature Biotech. 2015; 33:985–989.
4.
FarboudB, et al.Enhanced genome editing with Cas9 ribonucleoprotein in diverse cells and organisms. J. Visualized Experiments: JoVE 2018; 135. DOI:10.3791/57350.
