Abstract
Objective: To evaluate the in vivo wound modulating effects of a new biologic tissue adhesive. Background Data: Biological tissue glue can serve as an important adjunct technology in the treatment of a number of ocular conditions, including closure of corneal wounds. These experiments were performed to evaluate the wound healing effects of a new photodynamic biologic tissue glue (PBTG) in perforating rabbit corneal incisions. Methods: Perforating corneal wounds were created in 12 pigmented rabbits. Two perforating incisions were made 180° apart 2 mm from the limbus. One incision in each eye was closed with a single interrupted 10-0 nylon suture, and the other closed with PBTG. Rabbits were followed biomicroscopically and sacrificed at 1-, 3-, and 7-week intervals for histological evaluation of their corneal wounds. Results: There were no clinical signs of PBTG-related toxicity in any of the rabbits. Compared to control wounds, all glued corneas had greater epithelial filling, less stromal edema, and fewer inflammatory cells at the 1-, 3-, and 7-week stages and showed greater overall wound maturation. Conclusion: Our data suggest that PBTG is an effective and nontoxic tissue adhesive that accelerates normal corneal wound healing.
Get full access to this article
View all access options for this article.