Abstract
The optical laser setting for successful hemostatic transection of vessels using laser bipolar dissector (LBD) and the important factor in closure of the vessel stump were examined in abdominal aortas and mesenteric arteries of rabbit (n = 20). Interluminal bursting pressures (BP) were measured. Histopathological examinations were carried out by HE and EVG stainings. The BP immediately after transection at laser power of 10 (n = 5) and 13 W (n = 5) was above 346 mm Hg, and that of 15 W (n = 5) and 20 W (n = 5) was 280 and 208 mm Hg, respectively. The transected sites of vessels revealed well-apposed tissue welding of vascular wall that divided histologically into 4 areas: a carbonization area, a coagulation area, a conjunction area, and a tapering area. The welding of internal elastic lamina without fragmentation is histologically most important for closure of the vessel stump. An increase in the laser power from 10 to 20 W resulted in increased severity of histopathological deterioration of tissue connectivity (n = 10, P < 0.01) and in decreased bursting strength (n = 5, p < 0.01). Laser power of 10–13 W was the most suitable for hemostatic dissection of large vessels.
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