A major improvement in the generation of astrocytes directly from human fibroblasts will now facilitate the study of how aging impacts on astrocyte function and whether this contributes to neurodegenerative disorders.
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References
1.
CaiazzoM, GiannelliS, ValenteP, et al.Direct conversion of fibroblasts into functional astrocytes by defined transcription factors. Stem Cell Reports, 2015; 4(1):25–36; doi: 10.1016/j.stemcr.2014.12.002.
2.
CanalsI, GinistyA, QuistE, et al.Rapid and efficient induction of functional astrocytes from human pluripotent stem cells. Nat Methods, 2018; 15(9):693–696; doi: https://doi.org/10.1038/s41592-018-0103-2.
3.
Drouin-OuelletJ, LauS, BrattåsPL, et al.REST suppression mediates neural conversion of adult human fibroblasts via microRNA-dependent and -independent pathways. EMBO Mol Med, 2017; 9(8):1117–1131; doi: https://doi.org/10.15252/emmm.201607471.
4.
HabibN, McCabeC, MedinaS, et al.Disease-associated astrocytes in Alzheimer's disease and aging. Nat Neurosci, 2020; 23(6):701–706; doi: https://doi.org/10.1038/s41593-020-0624-8.
5.
KamTI, HinkleJT, DawsonTM, et al.Microglia and astrocyte dysfunction in Parkinson's disease. Neurobiol Dis, 2020; 144:105028; doi: https://doi.org/10.1016/j.nbd.2020.105028.
6.
LadewigJ, MertensJ, KesavanJ, et al.Small molecules enable highly efficient neuronal conversion of human fibroblasts. Nat Methods, 2012; 9(6):575–578; doi: 10.1038/nmeth.1972.
7.
MertensJ, PaquolaAC, KuM, et al.Directly reprogrammed human neurons retain aging-associated transcriptomic signatures and reveal age-related nucleocytoplasmic defects. Cell Stem Cell, 2015; 17(6):705–718; doi: 10/1016/j.stem.2015.09.001.
8.
MertensJ, ReidD, LauS, et al.Aging in a dish: iPSC-derived and directly induced neurons for studying brain aging and age-related neurodegenerative diseases. Annu Rev Genet, 2018; 52:271–293; doi: 10/1146/annurev-genet-120417-031534.
PangZP, YangN, VierbuchenT, et al.Induction of human neuronal cells by defined transcription factors. Nature, 2011;476(7359):220–223; doi: https://doi.org/10.1038/nature10202.
11.
QuistE, TrovatoF, AvalianiN, et al.Transcription factor-based direct conversion of human fibroblasts to functional astrocytes. Stem Cell Reports, 2022; 17(7):1620–1635; doi: 10/1016/j.stemcr.2022.05.015.
