To investigate the feasibility of β-tricalcium phosphate (TCP)/gelatine scaffold combined with allogeneic adipose-derived stem cells (ASCs) to repair hole shape defect, third-passage ASCs were seeded onto composite scaffolds to prepare an ASC-β-TCP/gelatine tissue-engineered bone to pack into the rabbit cavernous bone defects of experimental groups. In animal models, the bone defect area was completely filled and difficult to recognize in the experimental group at 12 weeks post-surgery by gross observation and radiographic examination. The average bone mineral density value of them was higher than that of the control group. Because of the biocompatibility with allogenic ASCs and the osteoconductivity of β-TCP/gelatine scaffolds, β-TCP/gelatine is suitable as a plastic scaffold for the ASC-seeded tissue-engineered bone to repair cavernous defects.
Get full access to this article
View all access options for this article.
References
1.
AryalR., ChenX.P., FangC., and HuY.C. (2014). Bone morphogenetic protein-2 and vascular endothelial growth factor in bone tissue regeneration: New insight and perspectives. Orthop. Surg. 6, 171–178.
2.
BachtiarE.W., AmirL.R., SuhardiP., and AbasB. (2016). Scaffold degradation during bone tissue reconstruction in Macaca nemestrina mandible. Interv. Med. Appl. Sci. 8, 77–81.
3.
BurrowK.L., HoylandJ.A., and RichardsonS.M. (2017). Human adipose-derived stem cells exhibit enhanced proliferative capacity and retain multipotency longer than donor-matched bone marrow mesenchymal stem cells during expansion in vitro. Stem Cells Int. 2017,2541275.
4.
CaoF., LiuT., XuY., XuD., and FengS. (2015). Culture and properties of adipose-derived mesenchymal stem cells: characteristics in vitro and immunosuppression in vivo. Int. J. Clin. Exp. Pathol. 8, 7694–7709.
5.
CarreiraA.C., LojudiceF.H., HalcsikE., NavarroR.D., SogayarM.C., and GranjeiroJ.M. (2014). Bone morphogenetic proteins: facts, challenges, and future perspectives. J. Dent. Res. 93, 335–345.
6.
DalyA.C., FreemanF.E., Gonzalez-FernandezT., CritchleyS.E., NultyJ., and KellyD.J. (2017). 3D bioprinting for cartilage and osteochondral tissue engineering. Adv. Healthc. Mater. 6.
7.
DanisovicL., OravcovaL., KrajciovaL., VarchulovaN.Z., BohacM., VargaI., and VojtassakJ. (2017). Effect of long-term culture on the biological and morphological characteristics of human adipose tissue-derived stem cells. J. Physiol. Pharmacol. 68, 149–158.
8.
DaviesO.G., CooperP.R., SheltonR.M., SmithA.J., and SchevenB.A. (2015). Isolation of adipose and bone marrow mesenchymal stem cells using CD29 and CD90 modifies their capacity for osteogenic and adipogenic differentiation. J. Tissue Eng. 6,2041731415592356.
9.
GhasroldashtM.M., Irfan-MaqsoodM., MatinM.M., BidkhoriH.R., Naderi-MeshkinH., MoradiA., and BahramiA.R. (2014). Mesenchymal stem cell based therapy for osteo-diseases. Cell Biol. Int. 38, 1081–1085.
10.
HikitaA., ChungU.I., HoshiK., and TakatoT. (2017). Bone regenerative medicine in oral and maxillofacial region using a three-dimensional printer. Tissue Eng. Part A, 23, 515–521.
11.
JiaL., PengZ., YuL., ChunxiaH., and DannaC. (2018). Repair of bone defects in rat radii with a composite of allogeneic adipose-derived stem cells and heterogeneous deproteinized bone. Stem Cell Res. Ther. 9,79.
12.
KanakarisN.K., and GiannoudisP.V. (2008). Clinical applications of bone morphogenetic proteins: Current evidence. J. Surg. Orthop. Adv. 17, 133–146.
13.
LiaoH.T., and ChenC.T. (2014). Osteogenic potential: Comparison between bone marrow and adipose-derived mesenchymal stem cells. World J. Stem Cells, 6, 288–295.
14.
LiuJ.T., ZhangF., GaoZ.C., NiuB.B., LiY.H., and HeX.J. (2017). Development and application of artificial vertebral body. Zhongguo Gu Shang, 30, 1157–1164.
15.
LoebelC., and BurdickJ.A. (2018). Engineering stem and stromal cell therapies for musculoskeletal tissue repair. Cell Stem Cell, 22, 325–339.
16.
MankaniM.H., KuznetsovS.A., FowlerB., KingmanA., and RobeyP.G. (2001). In vivo bone formation by human bone marrow stromal cells: effect of carrier particle size and shape. Biotechnol. Bioeng. 72, 96–107.
17.
Meimandi-PariziA., OryanA., and GholipourH. (2017). Healing potential of nanohydroxyapatite, gelatin, and fibrin-platelet glue combination as tissue engineered scaffolds in radial bone defects of rats. Connect. Tissue Res. 16, 1–13.
18.
OliveiraH.L., Da RosaW.L.O., Cuevas-SuárezC.E., CarreñoN.L.V., da SilvaA.F., GuimT.N., DellagostinO.A., and PivaE. (2017). Histological evaluation of bone repair with hydroxyapatite: A systematic review. Calcif. Tissue Int. 101, 341–354.
19.
OryanA., AlidadiS., Bigham-SadeghA., and MoshiriA. (2016). Comparative study on the role of gelatin, chitosan and their combination as tissue engineered scaffolds on healing and regeneration of critical sized bone defects: An in vivo study. J. Mater. Sci. Mater. Med. 27,155.
20.
OryanA., AlidadiS., Bigham-SadeghA., MoshiriA., and KamaliA. (2017). Effectiveness of tissue engineered chitosan-gelatin composite scaffold loaded with human platelet gel in regeneration of critical sized radial bone defect in rat. J. Control. Release, 254, 65–74.
21.
PapavasiliouA., StantonJ., SinhaP., ForderJ., and SkyrmeA. (2007). The complexity of seat belt injuries including spinal injury in the pediatric population: A case report of a 6-year-old boy and the literature review. Eur. J. Emerg. Med. 14, 180–183.
22.
PauloM.J.E., Dos SantosM.A., CimattiB., GavaN.F., RibertoM., and EngelE.E. (2017). Osteointegration of porous absorbable bone substitutes: A systematic review of the literature. Clinics (Sao Paulo), 72, 449–453.
23.
RappA.E., BindlR., HeilmannA., ErbacherA., MüllerI., BrennerR.E., and IgnatiusA. (2015). Systemic mesenchymal stem cell administration enhances bone formation in fracture repair but not load-induced bone formation. Eur. Cells Mater. 29, 22–34.
24.
ReynoldsJ.J., KhundkarR., BorianiS., WilliamsR., RhinesL.D., KawaharaN., WolinskyJ.P., GokaslanZ.L., and VargaP.P. (2016). Soft tissue and bone defect management in total sacrectomy for primary sacral tumors: A systematic review with expert recommendations. Spine (Phila Pa 1976) 41 Suppl, 20, S199–S204.
25.
SchneiderS., UngerM., van GriensvenM., and BalmayorE.R. (2017). Adipose-derived mesenchymal stem cells from liposuction and resected fat are feasible sources for regenerative medicine. Eur. J. Med. Res. 22,17.
26.
TajimaS., TobitaM., and MizunoH. (2017). Current status of bone regeneration using adipose-derived stem cells. Histol. Histopathol. 2,11942.
27.
ToyserkaniN.M., JørgensenM.G., TabatabaeifarS., JensenC.H., SheikhS.P., and SørensenJ.A. (2017). Concise review: A safety assessment of adipose-derived cell therapy in clinical trials: A systematic review of reported adverse events. Stem Cells Transl. Med. 6, 1786–1794.
28.
VanhatupaS., OjansivuM., AutioR., JuntunenM., and MiettinenS. (2015). Bone morphogenetic protein-2 induces donor-dependent osteogenic and adipogenic differentiation in human adipose stem cells. Stem Cells Transl. Med. 4, 1391–1402.
29.
WatsonL., EllimanS.J., and ColemanC.M. (2014). From isolation to implantation: A concise review of mesenchymal stem cell therapy in bone fracture repair. Stem Cell Res. Ther. 5,51.
30.
XuG., ZhangC., ZhouJ., HuangZ., and MengH. (2013). Advances in osteogenic mechanism and osteogenic effects of bone morphogenetic protein 6. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi, 27, 1144–1147.
