Long noncoding RNAs (lncRNAs) have been confirmed related to the occurrence and progress of multiple cancers, including cervical cancer nasopharyngeal carcinoma (NPC). This study focused on assessing GUSBP11 effects on NPC progression and exploring possible mechanisms.
Materials and Methods:
RT-qPCR was conducted for assessing GUSBP11 levels within NPC tissues and cells. CCK-8, colony formation, and Transwell were adopted for examining GUSBP11 impacts on NPC cell proliferation and cell metastasis. RT-qPCR analysis and dual-luciferase reporter assay were conducted for judging the expression interrelation of GUSBP11 and its potential target miR-1226-3p. The same methods were carried out for verifying the inhibiting influences of miR-1226-3p upregulation and its potential target TM9SF4.
Results:
GUSBP11 levels were upregulated within NPC tissues and cells. GUSBP11 downregulation repressed NPC cell proliferation and cell metastasis. In addition, GUSBP11 targeted and negatively regulated miR-1226-3p. Furthermore, miR-1226-3p targeted TM9SF4 and mediated GUSBP11's impacts on TM9SF4 levels. At last, the authors proved the critical role of the GUSBP11/miR-1226-3p/TM9SF4 axis in regulating NPC progression.
Conclusion:
These findings indicate that downregulation of GUSBP11 alleviates NPC development by regulating the miR-1226-3p/TM9SF4 axis.
Get full access to this article
View all access options for this article.
References
1.
TangLL, ChenWQ, XueWQ, et al.Global trends in incidence and mortality of nasopharyngeal carcinoma. Cancer Lett, 2016; 374:22.
2.
TorreLA, BrayF, SiegelRL, et al.Global cancer statistics, 2012. CA Cancer J Clin, 2015; 65:87.
3.
LiuZ, ChenY, SuY, et al.Nasopharyngeal carcinoma: Clinical achievements and considerations among treatment options. Front Oncol, 2021; 11:635737.
4.
LeeAW, FooW, LawSC, et al.Nasopharyngeal carcinoma: Presenting symptoms and duration before diagnosis. Hong Kong Med J, 1997; 3:355.
5.
KangY, HeW, RenC, et al.Advances in targeted therapy mainly based on signal pathways for nasopharyngeal carcinoma. Signal Transduct Target Ther, 2020; 5:245.
6.
ZhuJ, KongF, XingL, et al.Prognostic and clinicopathological value of long non-coding RNA XIST in cancer. Clin Chim Acta, 2018; 479:43.
7.
BoH, GongZ, ZhangW, et al.Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma. Oncotarget, 2015; 6:20404.
ZouZW, MaC, MedoroL, et al.LncRNA ANRIL is up-regulated in nasopharyngeal carcinoma and promotes the cancer progression via increasing proliferation, reprograming cell glucose metabolism and inducing side-population stem-like cancer cells. Oncotarget, 2016; 7:61741.
10.
NieGH, LiZ, DuanHF, et al.lncRNA C22orf32-1 contributes to the tumorigenesis of nasopharyngeal carcinoma. Oncol Lett, 2017; 13:4487.
11.
ZhengR, LiangJ, LuJ, et al.Genome-wide long non-coding RNAs identified a panel of novel plasma biomarkers for gastric cancer diagnosis. Gastric Cancer, 2019; 22:731.
12.
CaoW, LiuJN, LiuZ, et al.A three-lncRNA signature derived from the Atlas of ncRNA in cancer (TANRIC) database predicts the survival of patients with head and neck squamous cell carcinoma. Oral Oncol, 2017; 65:94.
13.
ZhouY, WangZ, ChenX, et al.Identification of differentially expressed miRNAs and mRNAs in synovial of osteoarthritis via RNA-sequencing. BMC Med Genet, 2020; 21:46.
14.
LaiXN, LiJ, TangLB, et al.MiRNAs and LncRNAs: Dual Roles in TGF-beta signaling-regulated metastasis in lung cancer. Int J Mol Sci, 2020; 21:1193.
15.
E Z, LiC, XiangY. LncRNA FOXD3-AS1/miR-135a-5p function in nasopharyngeal carcinoma cells. Open Med (Wars), 2020; 15:1193.
16.
ChenX, TanW, LiW, et al.miR-1226-3p promotes sorafenib sensitivity of hepatocellular carcinoma via downregulation of DUSP4 expression. J Cancer, 2019; 10:2745.
17.
LiY, SongD, AnT, et al.MicroRNA-1226-3p has a tumor-promoting role in osteosarcoma. Oncol Lett, 2021; 21:474.
18.
ChenX, YueB, ZhangC, et al.MiR-130a-3p inhibits the viability, proliferation, invasion, and cell cycle, and promotes apoptosis of nasopharyngeal carcinoma cells by suppressing BACH2 expression. Biosci Rep, 2017; 37:BSR20160576.
19.
CaoZ, ChenH, MeiX, LiX. Silencing of NACC1 inhibits the proliferation, migration and invasion of nasopharyngeal carcinoma cells via regulating the AKT/mTOR signaling pathway. Oncol Lett, 2021; 22:828.
20.
LivakKJ, SchmittgenTD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods, 2001; 25:402.
21.
SuiZ, SuiX. Long non-coding RNA TMPO-AS1 promotes cell proliferation, migration, invasion and epithelial-to-mesenchymal transition in gallbladder carcinoma by regulating the microRNA-1179/E2F2 axis. Oncol Lett, 2021; 22:855.
22.
ZhangL, WangY, ZhangL, et al.LINC01006 promotes cell proliferation and metastasis in pancreatic cancer via miR-2682-5p/HOXB8 axis. Cancer Cell Int, 2019; 19:320.
23.
ZhangM, ZhangM, LiR, et al.Melatonin sensitizes esophageal cancer cells to 5fluorouracil via promotion of apoptosis by regulating EZH2 expression. Oncol Rep, 2021; 45:22.
24.
ZhaoX. miR-1258 regulates cell proliferation and cell cycle to inhibit the progression of breast cancer by targeting E2F1. Biomed Res Int, 2020; 2020:1480819.
25.
ZhengZQ, LiZX, ZhouGQ, et al.Long noncoding RNA FAM225A promotes nasopharyngeal carcinoma tumorigenesis and metastasis by acting as ceRNA to Sponge miR-590-3p/miR-1275 and Upregulate ITGB3. Cancer Res, 2019; 79:4612.
26.
LiW, WuX, SheW. LncRNA POU3F3 promotes cancer cell migration and invasion in nasopharyngeal carcinoma by up-regulating TGF-beta1. Biosci Rep, 2019; 39:BSR20181632.
27.
MaDD, YuanLL, LinLQ. LncRNA HOTAIR contributes to the tumorigenesis of nasopharyngeal carcinoma via up-regulating FASN. Eur Rev Med Pharmacol Sci, 2017; 21:5143.
28.
LiuY, TaoZ, QuJ, et al.Long non-coding RNA PCAT7 regulates ELF2 signaling through inhibition of miR-134-5p in nasopharyngeal carcinoma. Biochem Biophys Res Commun, 2017; 491:374.
29.
LianY, XiongF, YangL, et al.Long non-coding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway. J Exp Clin Cancer Res, 2018; 37:253.
30.
LanX, LiuX. LncRNA SNHG1 functions as a ceRNA to antagonize the effect of miR-145a-5p on the down-regulation of NUAK1 in nasopharyngeal carcinoma cell. J Cell Mol Med, 2019; 23:2351.
31.
BenghezalM, CornillonS, GebbieL, et al.Synergistic control of cellular adhesion by transmembrane 9 proteins. Mol Biol Cell, 2003; 14:2890.
32.
BergeretE, PerrinJ, WilliamsM, et al.TM9SF4 is required for Drosophila cellular immunity via cell adhesion and phagocytosis. J Cell Sci, 2008; 121:3325.
33.
SunL, MengZ, ZhuY, et al.TM9SF4 is a novel factor promoting autophagic flux under amino acid starvation. Cell Death Differ, 2018; 25:368.
34.
LozuponeF, PerdicchioM, BrambillaD, et al.The human homologue of Dictyostelium discoideum phg1A is expressed by human metastatic melanoma cells. EMBO Rep, 2009; 10:1348.
35.
ZhuY, XieM, MengZ, et al.Knockdown of TM9SF4 boosts ER stress to trigger cell death of chemoresistant breast cancer cells. Oncogene, 2019; 38:5778.
36.
LozuponeF, BorghiM, MarzoliF, et al.TM9SF4 is a novel V-ATPase-interacting protein that modulates tumor pH alterations associated with drug resistance and invasiveness of colon cancer cells. Oncogene, 2015; 34:5163.
