Abstract
Objective:
As a wide-ranging tumor arising from the central nervous system, the exact cause of glioma continues to allude researchers and healthcare providers. This study conducted a bioinformatics approach to perform a comparative analysis among astrocytoma, ependymoma, and oligodendroglioma. Unique disease genes of the three aforementioned glioma subtypes were screened throughout various databases.
Methods:
Genomic functional annotations for unique disease genes and direct interacting genes were obtained with Gene Ontology (GO). The optimal GO terms were selected and the applicable genes were subsequently regarded as type A genes. Differentially expressed genes between the three glioma subtypes were screened as type B genes from the Gene Expression Omnibus. R language was employed in the gene co-expression network analyses between three subtypes, among which the genes with varying correlations were selected as type C genes and genes with different functional modules were selected as type D genes. The common genes among type A–D genes were concluded to be key genes (DISCI and TREM2) followed by the construction of the overexpressed and silenced vectors, as well as cell transfection, MTT assay, scratch test, and Transwell assay.
Results:
In comparison to ependymoma, DISCI exhibited high expression in astrocytoma and oligodendroglioma; TREM2 was upregulated in astrocytoma, but downregulated in oligodendroglioma. DISC1 was found to enhance growth, proliferation, migration, and invasion in both the astrocytoma (U343) and oligodendroglioma cell lines (Hs683), but a negligible effect on the ependymoma cell line (KNS-42). Furthermore, TREM2 had an enhanced impact on U343 cell growth, proliferation, migration, and invasion, while it was observed to be an insignificant contributor regarding the Hs683 cells.
Conclusions:
The key findings of the study provide evidence indicating that DISCI exhibits varying expression levels between astrocytoma and ependymoma, as well as between oligodendroglioma and ependymoma; TREM2 was verified to be differently expressed between astrocytoma and oligodendroglioma.
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Supplementary Material
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