Inhibition of Lung Adenocarcinoma Transfected with Interleukin 28A Recombinant Adenovirus (Ad-mIFN-λ 2 ) In Vivo

Abstract

Type III interferon (IFN-λ) is a novel member of the interferon family, which preferentially promotes antiviral responses from epithelial cells and cooperates with type I IFNs in the clearance of viral infections. However, the effect of mIFN-λ₂ to the LA795 lung adenocarcinoma cell is largely unknown. In this study, we transfected Ad-mIFN-λ₂ vector into LA795 tumor-bearing mice to explore the effect of mIFN-λ₂ on the proliferation of LA795 lung adenocarcinoma cell and on the immune response of the mice. Transfected by Ad-mIFN-λ₂ vector, a significant decrease in the tumor growth, the subcutaneous tumor necrosis, cystic degeneration, and tumor apoptosis were more evident; at the same time, mIFN-λ₂ protein and gene were significantly more expressed. And, flow cytometry analysis suggested that CD3⁺CD4⁺, CD3⁺CD8⁺, and NK (CD3⁻CD49⁺) cells were all significantly increased after transfected by Ad-mIFN-λ₂. The study demonstrated that recombinant Ad-mIFN-λ₂ transfection effectively inhibited the growth of LA795 lung adenocarcinoma cell, which may work through inducing apoptosis of tumor cell and regulating cell immune response.