Sage Journals: Discover world-class research

Abstract

Purpose:

This study investigates whether 8.8 mT static magnetic fields (SMFs) can enhance the killing potency of cisplatin (DDP) on human leukemic cells (K562).

Methods:

The cell proliferation, cell cycle distribution, DNA damage, and the change in cell surface ultrastructure after K562 cells were exposed to 8.8 mT SMFs with or without DDP were analyzed.

Results:

The results show that SMFs enhanced the killing effect of DDP on K562 cells, reducing the efficient killing concentration of DDP on K562 cells from 20 to 10 μg/mL. Atomic force microscope observation showed that the cell surface ultrastructure was altered. The results of fluorescence-activated cell sorting analysis indicated that K562 cells treated with SMF plus DDP were arrested at the S phase. The SMF exposure induced DNA to become thicker than controls, and breakage of DNA occurred in the DDP group; however, DNA breakage was increased in the SMF + DDP group.

Conclusions:

The results show that SMFs enhanced the anticancer effect of DDP on K562 cells. The mechanism correlated with the DNA damage model. This study also shows the potentiality of SMFs as an adjunctive treatment method for chemotherapy.

Get full access to this article

View all access options for this article.

References

Omote

, Hosokawa

, Komatsumoto

et al. Treatment of experimental tumors with a combination of a pulsing magnetic field and an antitumor drug. Jpn J Cancer Res, 1990; 81:956.

Sabo

, Mirossay

, Horovcak

et al. Effects of static magnetic field on human leukemic cell line HL-60. Bioelectrochemistry, 2002; 56:227.

Hannan

Jr. , Liang

, Allison

et al. In vitro cytotoxicity against human cancer cell lines during pulsed magnetic field exposure. Anticancer Res, 1994; 14:1517.

Hannan

Jr. , Liang

, Allison

et al. Chemotherapy of human carcinoma xenografts during pulsed magnetic field exposure. Anticancer Res, 1994; 14:1521.

Gray

, Frith

, Parker

. In vivo enhancement of chemotherapy with static electric or magnetic fields. Bioelectromagnetics, 2000; 21:575.

Chakkalakal

, Mollner

, Bogard

et al. Magnetic field induced inhibition of human osteosarcoma cells treated with adriamycin. Cancer Biochem Biophys, 1999; 17:89.

Jung

, Lippard

. Direct cellular responses to platinum-induced DNA damage. Chem Rev, 2007; 107:1387.

Suo

, Lippard

, Johnson

. Single d(GpG)/cis-diammineplatinum(II) adduct-induced inhibition of DNA polymerization. Biochemistry, 1999; 38:715.

Lozzio

, Lozzio

. Properties and usefulness of the original K-562 human myelogenous leukemia cell line. Leuk Res, 1979; 3:363.

10.

Mosmann

. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods, 1983; 65:55.

11.

Luo

, Qi

, Chen

et al. DNA damage induced by UV in K562 cells measured by comet assay. Carcinog Teratog Mutagen, 2006; 18:400.

12.

Helma

, Uhl

. A public domain image-analysis program for the single-cell gel-electrophoresis (comet) assay. Mutat Res, 2000; 466:9.

13.

Bowden

, Buckwalter

, McBride

et al. Tail profile: A more accurate system for analyzing DNA damage using the Comet assay. Mutat Res, 2003; 537:1.

14.

Konca

, Lankoff

, Banasik

et al. A cross-platform public domain PC image-analysis program for the comet assay. Mutat Res, 2003; 534:15.

15.

Hellman

, Vaghef

, Bostrom

. The concepts of tail moment and tail inertia in the single cell gel electrophoresis assay. Mutat Res, 1995; 336:123.

16.

Collins

. The comet assay for DNA damage and repair: Principles, applications, and limitations. Mol Biotechnol, 2004; 26:249.

17.

Zhuang

, Qi

, Sun

et al. Real time observation on cells in different fixed conditions and living cells in buffer solution by AFM. Acta Biophys Sin, 2005; 21:345.

18.

Zhang

, Qi

, Li

et al. Visualization of cellular DNA crosslinks by atomic force microscopy. Scanning, 2009; 31:75.

19.

Tenuzzo

, Chionna

, Panzarini

et al. Biological effects of 6 mT static magnetic fields: A comparative study in different cell types. Bioelectromagnetics, 2006; 27:560.

20.

Budiman

, Bierbach

, Alexander

. DNA minor groove adducts formed by a platinum-acridine conjugate inhibit association of tata-binding protein with its cognate sequence. Biochemistry, 2005; 44:11262.

21.

Miyakoshi

, Horiuchi

, Nakahara

et al. Magnetic fields generated by an induction heating (IH) cook top do not cause genotoxicity in vitro. Bioelectromagnetics, 2007; 28:529.

22.

Zmyslony

, Palus

, Dziubaltowska

et al. Effects of in vitro exposure to power frequency magnetic fields on UV-induced DNA damage of rat lymphocytes. Bioelectromagnetics, 2004; 25:560.

23.

McNamee

, Bellier

, McLean

et al. DNA damage and apoptosis in the immature mouse cerebellum after acute exposure to a 1 mT, 60 Hz magnetic field. Mutat Res, 2002; 513:121.

24.

Allen

, Bradbury

, Balhorn

. AFM analysis of DNA-protamine complexes bound to mica. Nucleic Acids Res, 1997; 25:2221.

25.

Lozzio

, Lozzio

. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood, 1975; 45:321.

26.

Naaman

, Vager

. New electronic and magnetic properties emerging from adsorption of organized organic layers. Phys Chem Chem Phys, 2006; 8:2217.

27.

Hamada

, Tsuruo

. Characterization of the ATPase activity of the Mr 170,000 to 180,000 membrane glycoprotein (P-glycoprotein) associated with multidrug resistance in K562/ADM cells. Cancer Res, 1988; 48:4926.

Static Magnetic Fields Enhanced the Potency of Cisplatin on K562 Cells

Abstract

Purpose:

Methods:

Results:

Conclusions:

Get full access to this article

References