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Abstract

Aim:

The aim of this study was to explore the inhibitory effects of Tanshinone II_A on the production of proinflammation cytokines in radiation-stimulated microglia.

Methods:

Microglia cells were treated with 2, 4, 8, 16, and 32 Gy of irradiation or sham-irradiated in the presence or absence of 1.0 μg/mL of Tanshinone II_A. The effects of Tanshinone II_A on radiation-induced proinflammatory cytokines were evaluated by real-time polymerase chain reaction; the expression level of nuclear factor (NF-κβ) p65 in cytoplasm and nucleus was measured by Western blot. Immunofluorescence staining and confocal microscopy analysis were applied to detect the expression of γ-H2AX and p65 postirradiation.

Results:

Radiation-induced release of proinflammatory cytokines in BV-2 cells was detectable after irradiation. Tanshinone II_A decreased the radiation-induced release of proinflammatory cytokines. Further, Western blotting showed that Tanshinone II_A could attenuate the nuclear translocation of (NF-κβ) p65 submit postirradiation. Immunofluorescence staining showed γ-H2AX foci formation with p65 translocation into the nucleus postirradiation.

Conclusions:

Our data indicated that Tanshinone II_A exerts anti-inflammatory properties by suppressing the transcription of proinflammatory cytokine genes that might be associated with the NF-κβ signaling pathway. It is postulated that irradiation causes immediate cellular reaction, and that double-strand breaks trigger the molecular response that leads to NF-κβ pathway activation.

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Anti-Inflammatory Effects of Tanshinone II A on Radiation-Induced Microglia BV-2 Cells Inflammatory Response

Abstract

Aim:

Methods:

Results:

Conclusions:

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References