Synthesis and Biodistribution of Peptide based 99mTc/186Re-MAGIPG-D612 Monoclonal Antibody in Nude Mice Bearing Colon Cancer Xenografts

Abstract

A new bifunctional chelating agent MAGIPG was synthesized in good yield using Boc-p-nitrophenylalanine as the starting material. ^99mTc-labeled-MAGIPG-D612 conjugates reactive with human colon cancer were prepared in 3% to 15% yield with a radiochemical purity of 63% to 97%. ¹⁸⁶Re-labeled MAGIPG-D612 conjugates were prepared with a 7% to 30% yield and a radiochemical purity of 82% to 98%. The biodistribution results demonstrate that these radioimmunoconjugates selectively localized in LS174T human colon cancer xenografts.