Considering the high consumption of caffeinated beverages and its skeletal effects after onset of osteoporosis, we aimed to evaluate low and moderate in vitro caffeine concentrations in ovariectomized rat osteoblastic cells.
Materials and Methods:
Cells were cultured with 1 mM (OVXCAF1) and 3 mM (OVXCAF3) caffeine to assess proliferation, functional activity, and bone marker gene expression.
Results:
Cell proliferation in OVXCAF1 was similar to C and OVX after 10 and 14 days; OVXCAF3 showed a decrease after all periods. OVXCAF1 had a late increase of in situ ALP and a decrease in OVXCAF3. Mineralization was similar between C and OVXCAF1, with a decrease for OVXCAF3. Genes Alp and Bsp had a higher expression for OVXCAF1 compared with OVXCAF3. Gene Runx2 was upregulated for OVXCAF1 and OVXCAF3 in both periods, whereas gene Bglap was upregulated for OVXCAF1 and downregulated for OVXCAF3.
Conclusions:
Low caffeine concentrations maintained functional activity and promoted the upregulation of bone marker genes of osteoblastic cells from osteoporotic rats.
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