The search for assisted reproduction techniques applied to the conservation and even the genetic improvement of wild species is becoming increasingly common. Regarding conservation of male gametes from wild animals, although current advances are focused on cryopreservation, the development of protocols for sperm refrigeration seems to be underrated, despite its various advantages and applications. Therefore, this review aims to highlight the importance of short-term conservation of sperm from wild mammals, report the development of state-of-the-art refrigeration protocols for both ejaculated and epididymal sperm, and evaluate the challenges and prospects of their application.
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