Using cysteine and purslane extracts in extenders improved significantly the post-thaw sperm characteristics.
Sperm viability, DNA integrity, and mitochondrial activity demonstrate an improvement in post-thaw sperm.
Malondialdehyde production was decreased based on the positive effects of treated extenders.
The obtained results demonstrate that supplementation of 50 μg/mL of purslane methanolic extract with cysteine to freezing extenders was significantly superior compared with other treatments.
This study determined the effects of purslane aqueous extract (PAE), purslane methanolic extract (PME), and purslane ethanolic extract (PEE) compared with cysteine on goat semen. The samples collected for evaluation were pooled and diluted with a basic Tris diluent 50 μg/mL of purslane aqueous, methanolic, or ethanolic extract alone and in combination with 10 mM cysteine. Results demonstrated significant improvements using treated diluents in total motility, viability, mitochondrial activity, and decreased malondialdehyde (MDA) level. Also, all of the combined treatments and PME50μg/mL resulted in higher progressive motility, live spermatozoa, and integrity of DNA and plasma membranes. The apoptotic and dead spermatozoa were decreased significantly using PME50μg/mL, cysteine10mM + PME50μg/mL, and cysteine10mM + PAE50μg/mL. DNA integrity in cysteine10mM + PME50μg/mL was demonstrated to be higher than cysteine and PEE50μg/mL. Moreover, higher viability was observed in cysteine10mM + PME50μg/mL compared with the PME50μg/mL, PAE50μg/mL, PEE50μg/mL, and cysteine. From comparison among all treatments, cysteine10mM + PME50μg/mL showed the highest percentages of total motility, mitochondrial activity, and the lowest percentages of MDA. In conclusion, inclusion of purslane extracts and cysteine into the extenders improved the quality of cryopreserved spermatozoa. However, the mixture of methanolic extract and cysteine was more beneficial among the various treatments.
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