The aim of this study was to analyze the effects of maternal smoking on the total antioxidant status (TAS) and the concentrations of vitamins A and E in human breastmilk.
Methodology:
The study group (n=20) comprised postpartum women who declared smoking more than five cigarettes per day (confirmed by urinalysis of the cotinine concentration). The control group included 25 nonsmoking postpartum women. Breastmilk samples were collected between day 30 and day 32 after delivery. TAS was determined by Rice-Evans and Miller method, whereas the amount of vitamins A and E was measured by high-performance liquid chromatography.
Results:
No significant differences were observed between breastmilk samples from smoking and nonsmoking mothers in terms of TAS and vitamin A and E concentrations. Additionally, no significant correlations were found between urinary cotinine and TAS (R=0.35, p=0.144) or vitamin A (R=0.14, p=0.571) and vitamin E (R=0.31, p=0.228) concentrations in breastmilk samples from smoking mothers.
Conclusions:
Maternal smoking is not reflected by decreased TAS and vitamin A and E concentrations in mature milk.
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References
1.
GoldmanAS, GoldblumRM, HansonLA. Anti-inflammatory systems in human milk. Adv Exp Med Biol, 1990; 262:69–76.
2.
BuescherES, McIlheranSM. Colostral antioxidants: Separation and characterization of two activities in human colostrum. J Pediatr Gastroenterol Nutr, 1992; 14:47–56.
3.
Imhoff-KunschB, SteinAD, VillalpandoSet al.Docosahexaenoic acid supplementation from mid-pregnancy to parturition influence breast milk fatty acid concentration at 1 month postpartum in Mexican women. J Nutr, 2011; 141:321–326.
4.
NakamoriM, NinhNX, IsomuraHet al.Nutritional status of lactating mothers and their breast milk concentration of iron, zinc and copper in rural Vietnam. J Nutr Sci Vitaminol (Tokyo), 2009; 55:338–345.
5.
KristiansenAL, LandeB, ØverbyNCet al.Factors associated with exclusive breast-feeding and breast-feeding in Norway. Public Health Nutr, 2010; 13:2087–2096.
6.
RebhanB, KohlhuberM, SchweglerUet al.Infant feeding practices and associated factors through the first 9 months of life in Bavaria, Germany. J Pediatr Gastroenterol Nutr, 2009; 49:467–473.
7.
MathesonI, RivrudGN. The effect of smoking on lactation and infantile colic. JAMA, 1989; 261:42–43.
8.
AgostoniC, MarangoniF, GrandiFet al.Earlier smoking habits are associated with higher serum lipids and lower milk fat and polyunsaturated fatty acid content in the first 6 months of lactation. Eur J Clin Nutr, 2003; 57:1466–1472.
9.
ChechlowskaM, AmbroszkiewiczJ, GajewskaJet al.The effect of tobacco smoking during pregnancy on plasma oxidant and antioxidant status in mothers and newborn. Eur J Obstet Gynecol Reprod Biol, 2011; 155:132–136.
10.
CodandabanyU. Erythrocyte lipid peroxidation and antioxidants in cigarette smokers. Cell Biochem Funct, 2000; 18:99–102.
11.
MorrowJD, FreiB, LongmireAWet al.Increase in circulating products of lipid peroxidation (F2-isoprostanes) in smokers. Smoking as a cause of oxidative damage. N Engl J Med, 1995; 332:1198–1203.
12.
MarangonK, DevarajS, JialalI. Measurement of protein carbonyls in plasma of smokers and in oxidized LDL by an ELISA. Clin Chem, 1999; 45:577–578.
13.
MezzettiA, LapennaD, PierdomenicoSDet al.Vitamins E, C and lipid peroxidation in plasma and arterial tissue of smokers and non-smokers. Atherosclerosis, 1995; 112:91–99.
14.
YildizL, KayaogluN, AksoyH. The changes of superoxide dismutase, catalase and glutathione peroxidase activities in erythrocytes of active and passive smokers. Clin Chem Lab Med, 2002; 40:612–615.
15.
PincemailJ, VanbelleS, DegruneFet al.Lifestyle behaviours and plasma vitamin C and β-carotene levels from the ELAN population (Liege, Belgium)J Nutr Metab, 2011; 2011:494370.
16.
Rice-EvansC, MillerNJ. Total antioxidant status in plasma and body fluids. Methods Enzymol, 1994; 234:279–293.
17.
JafféMZ. About the precipitation caused by pikrinic acid in normal urine and about a new reaction of creatinine. Physiol Chem, 1886; 10:391–400.
18.
RiboliE, HaleyNJ, TredanielJet al.Misclassification of smoking status among women in relation to exposure to environmental tobacco smoke. Eur Respir J, 1995; 8:285–290.
19.
ArgüellesS, MachadoMJ, AyalaAet al.Correlation between circulating biomarkers of oxidative stress of maternal and umbilical cord blood at birth. Free Radic Res, 2006; 40:565–570.
20.
AycicekA, ErelO, KocyigitA. Decreased total antioxidant capacity and increased oxidative stress in passive smoker infants and their mothers. Pediatr Int, 2005; 47:635–639.
21.
ErmisB, YildirimA, OrsRet al.Influence smoking on serum and milk malondialdehyde, superoxide dismutase, glutathione peroxidase, and antioxidant potential levels in mothers at the postpartum seventh day. Biol Trace Elem Res, 2005; 105:27–36.
22.
OrtegaRM, López-SobalerAM, QuintasMEet al.The influence of smoking on vitamin C status during the third trimester of pregnancy and on vitamin C levels in maternal milk. J Am Coll Nutr, 1998; 17:379–384.
23.
AycicekA, IpekA. Maternal active or passive smoking causes oxidative stress in cord blood. Eur J Pediatr, 2008; 167:81–85.
24.
Szlagatys-SidorkiewiczA, ZagierskiM, KorzonM. Total antioxidative status in colostrum. The influence of maternal smoking [in Polish]Med Wieku Rozwoj, 2005; 9:621–624.
25.
FrosaliS, Di SimplicioP, PerroneSet al.Glutathione recycling and antioxidant enzyme activities in erythrocytes of term and preterm newborns at birth. Biol Neonate, 2004; 85:188–194.
26.
RipaldaMJ, RudolphN, WongSL. Developmental patterns of antioxidant defense mechanisms in human erythrocytes. Pediatr Res, 1989; 26:366–369.
27.
AtkinsonJ, EpandRF, EpandRM. Tocopherols and tosotrienols in membranes: A critical review. Free Radic Biol Med, 2008; 44:739–764.
28.
KielyM, CoganP, KearneyPJet al.Relationship between smoking, dietary intakes and plasma levels of vitamin E and beta-carotene in matched maternal-cord pairs. Int J Vitam Nutr Res, 1999; 69:262–267.
29.
OrhonFS, UlukolB, KahyaDet al.The influence of maternal smoking on maternal and newborn oxidant and antioxidant status. Eur J Pediatr, 2009; 168:975–981.
30.
OrtegaRM, Lopez-SobalerAM, MartinezRMet al.Influence of smoking on vitamin E status during the third trimester of pregnancy and on breast-milk tocopherol concentrations in Spanish women. Am J Clin Nutr, 1998; 68:662–667.
31.
SilvaSS, RondóP, BergamaschiDet al.Smoking during pregnancy and plasma concentrations beta-carotene and alpha-tocopherol in the immediate postpartum period in Brazilian women. Int J Vitam Nutr Res, 2005; 75:235–241.
32.
BolisettyS, NaidooD, LuiKet al.Postnatal changes in maternal and neonatal plasma antioxidant vitamins and the influence of smoking. Arch Dis Child Fetal Neonatal Ed, 2002; 86:F36–F40.
33.
SchwarzKB, CoxJM, SharmaSet al.Prooxidant effects of maternal smoking and formula in newborn infants. J Pediatr Gastroenterol Nutr, 1997; 24:68–74.
