Meteoritic material accumulated on the surface of the anoxic early Earth during the Late Heavy Bombardment around 4.0 Gya and may have provided Earth's surface with extraterrestrial nutrients and energy sources. This research investigates the growth of an anaerobic microbial community from pond sediment on native and pyrolyzed (heat-treated) carbonaceous chondrite Cold Bokkeveld. The community was grown anaerobically in liquid media. Native Cold Bokkeveld supported the growth of a phylogenetically clustered subset of the original pond community by habitat filtering. The anaerobic community on meteorite was dominated by the Deltaproteobacteria Geobacteraceae and Desulfuromonadaceae. Members of these taxa are known to use elemental sulfur and ferric iron as electron acceptors, and organic compounds as electron donors. Pyrolyzed Cold Bokkeveld, however, was inhibitory to the growth of the microbial community. These results show that carbonaceous chondrites can support and select for a specific anaerobic microbial community, but that pyrolysis, for example by geothermal activity, could inhibit microbial growth and toxify the material. This research shows that extraterrestrial meteoritic material can shape the abundance and composition of anaerobic microbial ecosystems with implications for early Earth. These results also provide a basis to design anaerobic material processing of asteroidal material for future human settlement.
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References
1.
BartonHA (2015) Starving artists: bacterial oligotrophic heterotrophy in caves. In Microbial Life of Cave Systems, edited by A Summers Engel. De Gruyter, Berlin, pp 79–104.
2.
BegonM, HarperJL, and TownsendCR (1996) Ecology. Individuals, Populations and Communities, 3rd edition. Blackwell Scientific Publications, Oxford.
3.
BirdLJ, ColemanML, and NewmanDK (2013) Iron and copper act synergistically to delay anaerobic growth of bacteria. Appl Environ Microbiol, 79:3619–3627.
4.
BokulichNA, KaehlerBD, RideoutJR, et al. (2018) Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2's q2-feature-classifier plugin. Microbiome, 6:90.
5.
BolyenE, RideoutJR, DillonMR, et al. (2019) Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2. Nat Biotechnol, 37:852–857.
BratbakG and DundasI (1984) Bacterial dry matter content and biomass estimations. Appl Environ Microbiol, 48:755–757.
8.
BrowningLB, McSweenHYJr, and ZolenskyME (1996) Correlated alteration effects in CM carbonaceous chondrites. Geochim Cosmochim Acta, 60:2621–2633.
9.
BryantDE, GreenfieldD, WalshawRD, et al. (2013) Hydrothermal modification of the Sikhote-Alin iron meteorite under low pH geothermal environments. A plausibly prebiotic route to activated phosphorus on the early Earth. Geochim Cosmochim Acta, 109:90–112.
10.
CanfieldDE and RaiswellR (1999) The evolution of the sulfur cycle. Am J Sci, 299:697–723.
11.
ChybaC and SaganC (1992) Endogenous production, exogenous delivery and impact-shock synthesis of organic molecules: an inventory for the origins of life. Nature, 355:125–132.
12.
ClarkeKR and WarwickRM (1998) A taxonomic distinctness index and its statistical properties. J Appl Ecol, 35:523–531.
13.
ClarkeKR and WarwickRM (1999) The taxonomic distinctness measure of biodiversity: weighting of step lengths between hierarchical levels. Mar Ecol Prog Ser, 184:21–29.
14.
ClarkeKR and WarwickRM (2001) A further biodiversity index applicable to species lists: variation in taxonomic distinctness. Mar Ecol Prog Ser, 216:265–278.
15.
EatonAD, ClesceriLS, FransonMAH, et al., editors (2005) Standard Methods for the Examination of Water & Wastewater, 21st ed. American Public Health Association, Denver, CO.
16.
GronstalA, PearsonV, KapplerA, et al. (2009) Laboratory experiments on the weathering of iron meteorites and carbonaceous chondrites by iron-oxidizing bacteria. Meteorit Planet Sci, 44:233–247.
HendershotWH, LalandeH, and DuquetteM (1993) Soil reaction and exchangeable acidity. In Soil Sampling and Methods of Analysis, edited by MR Carter. Lewis Publishers, Boca Raton, FL, pp 141–146.
19.
Hermitage of Braid and Blackford Hill Local Nature Reserve Management Plan 2011–2021 (nd) Hermitage of Braid and Blackford Hill Local Nature Reserve Management Plan 2011–2021, Edinburgh.
20.
JenniskensP, WilsonMA, PackanD, et al. (2000) Meteors: a delivery mechanism of organic matter to the early Earth. Earth Moon Planets, 82–83:57–70.
21.
KatohK, MisawaK, KumaK, et al. (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res, 30:3059–3066.
22.
KeddyPA (1992) Assembly and response rules: two goals for predictive community ecology. J Veg Sci, 3:157–164.
23.
KerridgeJF (1985) Carbon, hydrogen and nitrogen in carbonaceous chondrites: abundances and isotopic compositions in bulk samples. Geochim Cosmochim Acta, 49:1707–1714.
24.
KitajimaF, NakamuraT, TakaokaN, et al. (2002) Evaluating the thermal metamorphism of CM chondrites by using the pyrolytic behavior of carbonaceous macromolecular matter. Geochim Cosmochim Acta, 66:163–172.
25.
KomiyaM, ShimoyamaA, and HaradaK (1993) Examination of organic compounds from insoluble organic matter isolated from some Antarctic carbonaceous chondrites by heating experiments. Geochim Cosmochim Acta, 57:907–914.
26.
KumpLR (2008) The rise of atmospheric oxygen. Nature, 451:277–278.
27.
LiP-E, LoC-C, AndersonJJ, et al. (2017) Enabling the democratization of the genomics revolution with a fully integrated web-based bioinformatics platform. Nucleic Acids Res, 45:67–80.
28.
Loferer-KrößbacherM, KlimaJ, and PsennerR (1998) Determination of bacterial cell dry mass by transmission electron microscopy and densitometric image analysis. Appl Environ Microbiol, 64:688–694.
29.
LoveSG and BrownleeDE (1993) A direct measurement of the terrestrial mass accretion rate of cosmic dust. Science, 262:550–553.
30.
LozuponeC and KnightR (2005) UniFrac: a new phylogenetic method for comparing microbial communities. Appl Environ Microbiol, 71:8228–8235.
31.
MartinsZ (2011) Organic chemistry of carbonaceous meteorites. Elements, 7:35–40.
32.
MautnerMN (1997) Biological potential of extraterrestrial materials-1. Nutrients in carbonaceous meteorites, and effects on biological growth. Planet Space Sci, 45:653–664.
33.
MautnerMN (2002) Planetary resources and astroecology. Planetary microcosm models of asteroid and meteorite interiors: electrolyte solutions and microbial growth— implications for space populations and panspermia. Astrobiology, 2:59–76.
34.
Mautner MN LeonardRL, and DeamerDW (1995) Meteorite organics in planetary environments: hydrothermal release, surface activity, and microbial utilization. Planet Space Sci, 43:139–147.
35.
Mautner MN ConnerAJ, KillhamK, et al. (1997) Biological potential of extraterrestrial materials: 2. Microbial and plant responses to nutrients in the Murchison carbonaceous meteorite. Icarus, 129:245–253.
36.
McSweenHY Jr (1979) Alteration in CM carbonaceous chondrites inferred from modal and chemical variations in matrix. Geochim Cosmochim Acta, 43:1761–1770.
37.
MillerET, FarineDR, and TrisosCH (2017) Phylogenetic community structure metrics and null models: a review with new methods and software. Ecography, 40:461–477.
38.
MunsiriP, BoydCE, and HajekBF (1995) Physical and chemical characteristics of bottom soil profiles in ponds at Auburn, Alabama, USA and a proposed system for describing pond soil horizons. J World Aquac Soc, 26:346–377.
39.
NaraokaH, ShimoyamaA, MatsubayaO, et al. (1997) Carbon isotopic with relevance compositions of Antarctic carbonaceous chondrites to the alteration and existence of organic matter. Geochem J, 31:155–168.
40.
OdingaES, WaigiMG, GuddaFO, et al. (2020) Occurrence, formation, environmental fate and risks of environmentally persistent free radicals in biochars. Environ Int, 134:105172.
41.
PasekMA, DworkinJP, and LaurettaDS (2007) A radical pathway for organic phosphorylation during schreibersite corrosion with implications for the origin of life. Geochim Cosmochim Acta, 71:1721–1736.
42.
PearsonVK, SephtonMA, FranchiIA, et al. (2006) Carbon and nitrogen in carbonaceous chondrites: elemental abundances and stable isotopic compositions. Meteorit Planet Sci, 41:1899–1918.
43.
PizzarelloS, CooperGW, and FlynnGJ (2006) The nature and distribution of the organic material in carbonaceous chondrites and interplanetary dust particles. In Meteorites and the Early Solar System II, edited by DS Lauretta and HY McSween Jr. University of Arizona Press, Tucson, pp 625–651.
44.
PontarpM, SjöstedtJ, and LundbergP (2013) Experimentally induced habitat filtering in marine bacterial communities. Mar Ecol Prog Ser, 477:77–86.
45.
PriceMN, DehalPS, and ArkinAP (2009) Fasttree: computing large minimum evolution trees with profiles instead of a distance matrix. Mol Biol Evol, 26:1641–1650.
46.
PringleCR and BealeGH (1960) Antigenic polymorphism in a wild population of Paramecium aurelia. Genetical Research, 1:62–68.
47.
RemusatL, DerenneS, RobertF, et al. (2005) New pyrolytic and spectroscopic data on Orgueil and Murchison insoluble organic matter: a different origin than soluble?. Geochim Cosmochim Acta, 69:3919–3932.
48.
RognesT, FlouriT, NicholsB, et al. (2016) VSEARCH: a versatile open source tool for metagenomics. PeerJ, 4:e2584.
49.
RubinAE (1997) Mineralogy of meteorite groups. Meteorit Planet Sci, 32:231–247.
SmoldersAJP, LamersLPM, LucassenECHET, et al. (2006) Internal eutrophication: how it works and what to do about it—a review. Chem Ecol, 22:93–111.
52.
SteeleA, GoddardDT, StapletonD, et al. (2000) Investigations into an unknown organism on the martian meteorite Allan Hills 84001. Meteorit Planet Sci, 35:237–241.
53.
TaitAW, GagenEJ, WilsonSA, et al. (2017) Microbial populations of stony meteorites: substrate controls on first colonizers. Front Microbiol, 8:1227.
54.
ThomasP, SekharAC, UpretiR, et al. (2015) Optimization of single plate-serial dilution spotting (SP-SDS) with sample anchoring as an assured method for bacterial and yeast CFU enumeration and single colony isolation from diverse samples. Biotechnol Rep, 8:45–55.
55.
VargasM, KashefiK, Blunt-HarrisEL, et al. (1998) Microbiological evidence for Fe(III) reduction on early Earth. Nature, 395:65–67.
56.
WaajenGWAM, FaassenEJ, and LürlingM (2014) Eutrophic urban ponds suffer from cyanobacterial blooms: Dutch examples. Environ Sci Pollut Res Int, 21:9983–9994.
57.
WatsonJS, SephtonMA, and GilmourI (2010) Thermochemolysis of the Murchison meteorite: identification of oxygen bound and occluded units in the organic macromolecule. Int J Astrobiol, 9:201–208.
58.
WebbCO, AckerlyDD, and KembelSW (2008) Phylocom: software for the analysis of phylogenetic community structure and trait evolution. Bioinformatics, 24:2098–2100.
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