Phenylalanine (Phe) is an amino acid that has been identified in carbonaceous meteorites; its formation mechanism in space is unknown, and its radioresistance has been the subject of investigation. This work aims at studying, in the laboratory, the Phe radiolysis by cosmic analogues. The Phe destruction rate, at 300 K, is measured for H, He, and N ion beam irradiation in the 0.5 to 2 kinetic MeV range. Fourier transform infrared (FTIR) spectroscopy was employed to monitor the molecular degradation as a function of fluence. The Phe apparent destruction cross–section, σapd, which includes radiolysis and sputtering processes, is determined to be proportional to the electronic stopping power, Se. The measured parameter D0 = 14.3 ± 2.2 eV/molec in the relationship, and = Se/D0 is interpreted as the mean absorbed dose necessary to dissociate or eject a Phe molecule. The Phe half-life in the interstellar medium is predicted to be about 10 million years, H+ ions the main destructive cosmic ray constituent.
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References
1.
AndradeDP, de BarrosAL, PillingS, et al. (2013) Chemical reactions induced in frozen formic acid by heavy ion cosmic rays. Month Not Royal Astron Soc, 430:787–796.
2.
BennettCJ, PirimC, and OrlandoTM (2013) Space-weathering of solar system bodies: A laboratory perspective. Chem Rev, 113:9086–9150.
3.
BesenbacherF, BøttigerJ, GraversenO, et al. (1982) The erosion of frozen argon by swift helium ions. Nucl Instr Methods Phys Res, 198:27.
4.
CallahanMP, SmithKE, CleavesHJ, et al. (2011) Carbonaceous meteorites contain a wide range of extraterrestrial nucleobases. Proc Natl Acad Sci USA, 108:13995–13998.
5.
ChrysochoosJ (1968) Pulse radiolysis of phenylalanine and tyrosine. Radiat Res, 33:465–479.
6.
da CostaCAP, and Da SilveiraEF (2019) Valine infrared absorbance at cryogenic temperatures. Low Temp Phys, 45:649–655.
7.
da CostaCA, MunizGSV, BoduchP, et al. (2020) Valine radiolysis by H+, He+, N+, and S15+ MeV ions. Int J Mol Sci, 21:1893.
8.
da CostaCAP, Souza-CorrêaJA, and da SilveiraEF (2021) Infrared analysis of Glycine dissociation by MeV ions and keV electrons. Monthly Not Royal Astron Soc, 502:2105–2119.
9.
DartoisE, AugéB, BoduchP, et al. (2015) Heavy ion irradiation of crystalline water ice-Cosmic ray amorphisation cross-section and sputtering yield. Astron Astrophys, 576:A125.
10.
De BarrosALF, BordaloVSDE, DuarteES, et al. (2011) Cosmic ray impact on astrophysical ices: laboratory studies on heavy ion irradiation of methane. Astron Astrophys, 531:A160.
11.
DuarteES, DomarackaA, BoduchP, et al. (2010) Laboratory simulation of heavy-ion cosmic-ray interaction with condensed CO. Astron Astrophys, 512:A71.
12.
ElsilaJE, GlavinDP, and DworkinJP (2009) Cometary glycine detected in samples returned by Stardust. Meteorit Planet Sci, 44:1323–1330.
13.
FamáM, ShiJ, and BaragiolaRA (2008) Sputtering of ice by low-energy ions. Surf Sci, 602:156–161.
14.
GeorgeJ, LaveK, WiedenbeckM, et al. (2009) Astrophys J 698:1666.
15.
GerakinesPA, HudsonRL, MooreMH, et al. (2012) In situ measurements of the radiation stability of amino acids at 15–140 K. Icarus, 220:647–659.
16.
GlavinDP, AubreyAD, CallahanMP, et al. (2010) Extraterrestrial amino acids in the Almahata Sitta meteorite. Meteorit Planet Sci, 45:1695–1709.
17.
GuthierW, BeckerO, Della NegraS, et al. (1983) Secondary ion emission of phenylalanine as function of primary ion charge. Int J Mass Spectr Ion Phys, 53:185–200.
18.
HenningT and SemenovD (2013) Chemistry in protoplanetary disks. Chem Rev, 113:9016–9042.
19.
HernándezB, PflügerF, AdenierA, et al. (2010) Vibrational analysis of amino acids and short peptides in hydrated media. VIII. Amino acids with aromatic side chains: L-phenylalanine, L-tyrosine, and L-tryptophan. J Phys Chem B, 114:15319–15330.
20.
JohnsonRE (1996) Sputtering of ices in the outer solar system. Rev Modern Phys, 68:305.
21.
JursaAS (1985) Handbook of Geophysics and the Space Environment, 4th ed., Air Force Geophysics Laboratory, Hanscom AFB, MA.
22.
KhawasB (1985) X-ray study of L-phenylalanine dimorph and D-tyrptophane. Indian J Phys A, 59:219–226.
23.
KrajnikP, QuintRM, SolarS, et al. (1995) Influence of temperature and oxygen concentration on the radiation induced oxidation of phenylalanine. Zeitschrift für Naturforschung A, 50:864–870.
24.
LeiteCB, da SilveiraEF, JeronymoJMF, et al. (1992) Secondary-ion emission from phenylalanine induced by atomic and molecular MeV ion beams. Phys Rev B, 45:12218.
25.
LemoineP, Roy-LachapelleA, PrévostM, et al. (2013) Ultra-fast analysis of anatoxin-A using laser diode thermal desorption-atmospheric pressure chemical ionization-tandem mass spectrometry: validation and resolution from phenylalanine. Toxicon, 61:165–174.
26.
LudwigN, KusumotoT, GalindoC, et al. (2018) Radiolysis of phenylalanine in solution with Bragg-Peak energy protons. Radiat Meas, 116:55–59.
27.
MaryMB, SasirekhaV, and RamakrishnanV (2005) Laser raman and infrared spectral studies of DL- phenylalaninium nitrate. Spectrochim Acta Part A, 62:446–452.
28.
MaskosZ, RushJD, and KoppenolWH (1992) The hydroxylation of phenylalanine and tyrosine: a comparison with salicylate and tryptophan. Archive Biochem Biophys, 296:521–529.
29.
McKeeCF and OstrikerEC (2007) Theory of star formation. Annu Rev Astron Astrophys, 45:565–687.
30.
MeyerJP, DruryLOC, and EllisonDC (1998). A cosmic-ray composition controlled by volatility and A/Q ratio. SNR shock acceleration of gas and dust. Advan Comp Exp Mission. 179–201.
31.
MummaMJ and CharnleySB (2011) The chemical composition of comets—emerging taxonomies and natal heritage. Annu Rev Astron Astrophys, 49:471–524.
32.
NuevoM, MatereseCK, and SandfordSA (2014) The photochemistry of pyrimidine in realistic astrophysical ices and the production of nucleobases. Astrophys J, 793, doi:10.1088/0004-637X/793/2/125.
33.
ObaY, TakanoY, NaraokaH, et al. (2019) Nucleobase synthesis in interstellar ices. Nat Commun, 10:4413.
34.
OlsztynskaS, KomorowskaM, VrielynckL, et al. (2001) Vibrational spectroscopic study of L- phenylalanine: effect of pH. Appl Spectrosc, 55:901–907.
35.
OrzechowskaGE, GoguenJD, JohnsonPV, et al. (2007) Ultraviolet photolysis of amino acids in a 100 K water ice matrix: application to the outer Solar System bodies. Icarus, 187: 584–591.
36.
PereiraRC, de BarrosALF, da CostaCAP, et al. (2020) Ion irradiation of acetylene ice in the ISM and the outer Solar system: laboratory simulations. Month Not Royal Astronom Soc, 495:40–57.
37.
PillingS, MendesLAV, BordaloV, et al. (2013) The influence of crystallinity degree on the glycine decomposition induced by 1 MeV proton bombardment in space analog conditions. Astrobiology, 13:79–91.
38.
PortugalW, PillingS, BoduchP, RothardH, and AndradeDP (2014) Radiolysis of amino acids by heavy and energetic cosmic ray analogues in simulated space environments: α-glycine zwitterion form. Month Notices Royal Astronom Soc, 441:3209–3225.
39.
QuiricoE, MorozLV, SchmittB, et al. (2016) Refractory and semi- volatile organics at the surface of comet 67P/Churyumov-Gerasimenko: insights from the VIRTIS/Rosetta imaging spectrometer. Icarus, 272:32–47.
40.
RajkumarBJ and RamakrishnanV (2002) Infrared and laser Raman studies of L-phenylalanine L- phenylalaninium perchlorate and bis (DL-phenylalaninium) sulphate monohydrate. Spectrochim Acta Part A, 58:1923–1934.
41.
SalehpourM, HåkassonP, and SundqvistB (1984) Damage cross sections for fast heavy ion induced desorption of biomolecules. Nucl Instrum Methods Phys Res B, 2:752–756.
42.
SandfordSA, AléonJ, O'd AlexandeCM, et al. (2006) Organics captured from Comet 81P/Wild 2 by the Stardust spacecraft. Science, 314:1720–1724.
43.
SchouJ (1987) Sputtering of frozen gases. Nucl Instrum Methods Phys Res B. 27:188–200.
44.
ShenCJ, GreenbergJM, SchutteWA, and Van DishoeckEF (2004) Cosmic ray induced explosive chemical desorption in dense clouds. Astron Astrophys, 415:203–215.
45.
SigmundP (1987) Mechanisms and theory of physical sputtering by particle impact. Nucl Instrum Methods Phys Res B, 27:1–20.
46.
Souza-CorrêaJA, da CostaCAP, and da SilveiraEF (2019) Compaction and destruction cross-sections for α-glycine from radiolysis process via 1.0 keV electron beam as a function of temperature. Astrobiology, 19:1123–1138.
47.
van DishoeckEF, HerbstE, and NeufeldDA (2013) Interstellar water chemistry: from laboratory to observations. Chem Rev, 113:9043–9085.
48.
Vignoli MunizGS, MejíaCF, MartinezR, et al. (2017) Radioresistance of adenine to cosmic rays. Astrobiology, 17:298–308.
49.
WangD, SchuchmannHP, and von SonntagC (1993) Phenylalanine: its˙ OH and SO4˙−-induced oxidation and decarboxylation. A Pulse Radiolysis and Product Analysis Study. Zeitschrift für Naturforschung B, 48:761–770.
50.
WebberWR, and YushakSM (1983). A measurement of the energy spectra and relative abundance of the cosmic-ray H and He isotopes over a broad energy range. Astrophys J, 275:391–404.
51.
XieQ, XuL, HuC, et al. (2021) Phonon anharmonicity of thermoelectric material HfTe5 studied by Raman spectroscopy. J Raman Spectrosc, 52:988–994.
52.
ZeidlerS, MutschkeH, and PoschT (2015) Temperature-dependent infrared optical constants of olivine and enstatite. Astrophys J, 798:125.
53.
ZeidlerS, PoschT, and MutschkeH (2013) Optical constants of refractory oxides at high temperatures- Mid-infrared properties of corundum, spinel, and α-quartz, potential carriers of the 13 μm feature. Astron Astrophys, 553:A81.
54.
ZieglerJF, ZieglerMD, and BiersackJP (2010) SRIM the stopping and range of ions in matter. Nucl Instrum Methods Phys Res B, 268:1818–1823.
