Interaction of Methylglyoxal and Hydrogen Sulfide in Rat Vascular Smooth Muscle Cells

Abstract

Hydrogen sulfide (H₂S) is a gasotransmitter with multifaceted physiological functions, including the regulation of glucose metabolism. Methylglyoxal (MG) is an intermediate of glucose metabolism and plays an important role in the pathogenesis of insulin resistance syndromes. In the present study, we investigated the effect of MG on H₂S synthesis and the interaction between these two endogenous substances. In cultured vascular smooth muscle cells (VSMCs), MG (10, 30, and 50 μM) significantly decreased cellular H₂S levels in a concentration-dependent manner, while H₂S donor, NaHS (30, 60, and 90 μM), significantly decreased cellular MG levels. The expression level and activity of H₂S-producing enzyme, cystathionine γ-lyase (CSE), were significantly decreased by MG treatment. NaHS (30–90 μM) significantly inhibited MG (10 or 30 μM)-induced ROS production. Cellular levels of GSH, cysteine, and homocysteine were also increased by MG or NaHS treatment. Furthermore, direct reaction of H₂S with MG in both concentration- and time-dependent manners were observed in in vitro incubations. In conclusion, MG regulates H₂S level in VSMCs by downregulating CSE protein expression and directly reacting with H₂S molecule. Interaction of MG with H₂S may be one of future directions for the studies on glucose metabolism and the development of insulin resistance syndromes. Antioxid. Redox Signal. 12, 1093–1100.