Does Leukotriene Affect Intracellular Glutathione Redox State in Cultured Human Airway Epithelial Cells?

Abstract

Leukotrienes (LTs) are one of the most important mediators in the pathophysiology of asthma. We measured the intracellular amounts of reduced glutathione (GSH) and oxidized glutathione (GSSG) in cultured human airway epithelial cells. LTC 4 affects the GSH/GSSG ratio by activating signals to increase interleukin-8 (IL-8) production. Pretreatment with a reducing agent, glutathione monochrome ester (GSH-OEt), and with a leukotriene receptor antagonist, montelukast, significantly suppressed LTC₄-induced time-dependent changes in the intracellular redox state, and also suppressed upregulation of IL-8 production by suppressing NF-κB activation. Our observations led to the hypothesis that LTC₄-induced oxidative stress is likely to contribute to amplification of airway inflammation.