Restricted accessResearch articleFirst published online 2025-03
Improving the Solubility of Algerian Olive Leaf Extract,Enhancing Antibacterial Activities,and Preserving Bovine Sperm Viability Through Optimized Cyclodextrins Encapsulation Process
Olive leaf extract (OLE) possesses potent antioxidant properties crucial for safeguarding sperm, especially during cryopreservation. However, its limited solubility in aqueous solutions hampers optimal protective effects. This study aimed to optimize the extraction technique for OLE and enhance its solubility through the utilization of cyclodextrins.
Methods:
The optimized extraction conditions were characterized, with a primary focus on solubility. Subsequently, the impact on both sperm motility protection and the antibacterial potential effect was assessed.
Results and Discussion:
The optimal conditions involved using 20 g of olive leaves with an agitation duration of 60 minutes, yielding 368.509 EAG/mg dry extract of phenolic compounds. Encapsulation of OLE in cyclodextrins not only demonstrated an enhanced antibacterial effect but also showed a significant positive influence on sperm motility after 48 hours of preservation.
Conclusion:
This study highlights the effectiveness of cyclodextrin preparation as a viable strategy to improve OLE solubility, offering significant protection to spermatozoa during cryopreservation, along with an enhanced antibacterial effect.
ErbayZ, IcierF. The importance and potential uses of olive leaves. Food Rev Int, 2010; 26(4):319–334.
3.
XieC, LiX, LuoX, et al.Release modulation and cytotoxicity of hydroxycamptothecin-loaded electrospunfibers with 2-hydroxypropyl-β-cyclodextrin inoculations. Int J Pharm, 2010; 391(1–2):55–64.
4.
BulottaS, CelanoM, LeporeSM, et al.Beneficial effects of the olive oil phenolic components oleuropein and hydroxytyrosol: Focus on protection against cardiovascular and metabolic diseases. J Transl Med, 2014; 12:219.
5.
PolyakovNE, LeshinaTV, KonovalovaTA, et al.Inclusion complexes of carotenoids with cyclodextrins: NMR, EPR, and optical studies. Free Radic Biol Med, 2004; 36(7):872–880.
6.
FatmiS, TaouzinetL, Lahiani-SkibaM, et al.New formulation and evaluation of camptothecin encapsulated and/or dispersed suppository. Anticancer Agents Med Chem, 2021; 21(9):1183–1190.
7.
LeeOH, LeeBG, LeeJ, et al.Assessment of phenolics-enriched extract and fractions of olive leaves and their antioxidant activities. Bioresour Technol, 2009; 100(23):6107–6113.
8.
RamfulD, TarnusE, AruomaOI, et al.Polyphenol composition, vitamin C content and antioxidant capacity of Mauritian citrus fruit pulps. Food Res Int, 2011; 44(7):2088–2099.
9.
Sze‐TaoKWC, SchrimpfJE, TeuberSS, et al.Effects of processing and storage on walnut (Juglans regia L.) tannins. J Sci Food Agric, 2001; 81(13):1215–1222.
AbdullahNA, ZullkifleeN, ZainiSNZ, et al.Phytochemicals, mineral contents, antioxidants, and antimicrobial activities of propolis produced by Brunei stingless bees Geniotrigonathoracica, Heterotrigonaitama, and Tetrigonabinghami. Saudi J Biol Sci, 2020; 27(11):2902—2911.
12.
Lopes-LutzD, AlvianoDS, AlvianoCS, et al.Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils. Phytochemistry, 2008; 69(8):1732–1738.
13.
AliabadiAM, DarsanakiRK, RokhiML, et al.Antimicrobial activity of olive leaf aqueous extract. Ann Biol Res, 2012; 3(8):4189–4191.
14.
EbrahimiA, MauryP, BergerM, et al.QTL mapping of seed-quality traits in sunflower recombinant inbred lines under different water regimes, Revue. Genome, 2008; 51(8):599–615.
15.
MiliauskasG, VenskutonisPR, Van-BeekTA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem, 2004; 85(2):231–237.
16.
LeeM, LeeS, ZadehAD, et al.Distinct sites in E-cadherin regulate different steps in drosophila tracheal tube fusion. FlyBase, 2003; 130(24):5989–5999.
17.
LeybrosJ, FrémeauxP. Extraction solide-liquide. aspects théoriques, Technique de l’ingénieur. (L.) Karst. Int J Pharma Bio, 1990; 2:609–615.
18.
TrabelsiK, TouirJ, Soulié-MarscheI, et al.Albian charophytes discovered in the kebar Formation (Central Tunisia): Palaeoecological and palaeobiogeographical implications. Annales de Paléontologie, 2010; 96(3):117–133.
19.
KfouryM, AuezovaL, Greige-GergesH, et al.Promising applications of cyclodextrins in food: Improvement of essential oils retention, controlled release and antiradical activity. Carbohydr Polym, 2015; 131:264–272.
20.
ŞahinS, SamlıR. Optimization of olive leaf extract obtained by ultrasound-assisted extraction with response surface methodology. Ultrason Sonochem, 2013; 20(1):595–602.
21.
FatmiS, BournineL, IguerouadaM, et al.Amorphous solid dispersion studies of camptothecin cyclodextrin inclusion complexes in PEG 6000. Pol Pharm Soc, 2015; 72:179–192.
22.
ToutouZ, FatmiS, ChibaniN, et al.Cyclodextrins propolis extract encapsulation: Physicochemical characterization, toxicity effect, and application on animal sperm cold preservation. Starch, 2023; 75:2300017.
23.
BisignanoG, TomainoA, CascioRL, et al.On the in vitro antimicrobial activity of oleuropein and hydroxytyrosol. J Pharm Pharmacol, 1999; 51(8):971–974.
24.
MarkinD, DuekL, BerdicevskyI. In vitro antimicrobial activity of olive leaves. Mycoses, 2003; 46(3–4):132–136.
25.
VenugopalR, LiuRH. Phytochemicals in diets for breast cancer prevention: The importance of resveratrol and ursolic acid. Food Sci Hum Wellness, 2012; 1(1):1–13.
26.
TaouzinetL, FatmiS, KhelloufA, et al.Drug release, stability and efficiency of vitamin E loaded in liposomes for bovine sperm protection in cryopreservation medium. Cryo Letters, 2022; 43(1):50–57.
