Uric acid is a potent antioxidant; its levels correlate with antioxidant capacity and cell longevity. There are limited data on the antioxidant activity of Uric acid during oxidative stress in erythrocytes. Therefore, this study aimed to assess the effect of Uric acid on erythrocytes exposed to hydrogen peroxide.
Materials and Methods:
Erythrocytes were incubated with 2 mM H2O2 for 2 hours, followed by 5 mM Uric acid for 48 hours. They were grouped as (1) Controls (C)-without H2O2 or Uric acid; (2) Control+Free Radical (C+FR)-with H2O2; (3) Uric acid (U)-with Uric acid; (4) Uric acid+Free Radical (U+FR)-with Uric acid and H2O2. Hemoglobin, oxidative stress, and antioxidant markers were analyzed in the processed erythrocytes.
Results:
Superoxide dismutase activity and glutathione levels were elevated in Uric acid and U+FR. Catalase activity was higher in the Uric acid group than in the C+FR group. Protein sulfhydryls increased in the C+FR group. Uric acid and Nitrite levels increased in C+FR. Protein Carbonyls, advanced oxidation protein products, conjugate dienes, thiobarbituric acid reactive substances, and superoxides showed similar variations with Controls.
Conclusion:
Uric acid activated the antioxidant defenses and decreased the reactive species. Therefore Uric acid has been beneficial during H2O2-induced oxidative stress.
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