Genetic Analysis of HIV Type 1 env Gene in Cerebrospinal Fluid and Plasma of Infected Chinese Paid Blood Donors

Abstract

HIV infection in the central nervous system (CNS) can progress to AIDS dementia complex (ADC). Currently, the HIV-1 env gene in the CNS of infected Chinese paid blood donors (PBDs) has not been well characterized. In the study, the C2-V5 regions of the HIV env gene were cloned and sequenced from both cerebrospinal fluid (CSF) and plasma samples of six HIV-infected Chinese PBDs. Sequence analysis revealed that the sequences from Henan province clustered closely with subtypes B′ and B, and the levels of diversity from the CNS were significantly lower than those from blood (p<0.0001). In addition, all viral quasispecies from CNS use CCR5 as the coreceptor. These data provide valuable information on HIV pathogenesis in the CSF and plasma of infected Chinese PBDs, and our findings could enhance insights into HIV-associated neurological disease.