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Abstract

To monitor the mitochondrial transmembrane potential (ΔΨm), peripheral blood mononuclear cells (PBMCs) from 18 HIV-uninfected individuals completing a 4-week course of HIV postexposure prophylaxis (PEP) with emtricitabine/tenofovir and lopinavir/ritonavir were analyzed. ΔΨm was assessed by FACS analysis after staining with JC-1 using a mixed effects regression analysis. Apoptosis was determined by Annexin-V-FITC and propidium iodide staining. ΔΨm decreased significantly during HIV-PEP (p=0.0015 for the linear term, p=0.0039 for the quadratic term of days on PEP) and normalized several weeks after stopping antiretrovirals. The apoptosis rates did not change significantly (p=0.42). Even newer drugs such as tenofovir and emtricitabine may cause side effects due to mitochondrial dysfunction.

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Short Communication: Decrease in Mitochondrial Transmembrane Potential in Peripheral Blood Mononuclear Cells of HIV-Uninfected Subjects Undergoing HIV Postexposure Prophylaxis

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