Morphological,Histochemical,and Immunocytochemical Study of CO 2 and Er:YAG Laser Effect on Oral Soft Tissues

Abstract

Objective: The purpose of this study was to investigate the morphological, histochemical, and immunocytochemical changes of the oral mucosa after CO₂ or Er:YAG laser irradiation. Background Data: There have been no comparative reports on CO₂ and Er:YAG laser effects on human oral soft tissues. Materials and Methods: Tissue preservation was studied in 40 oral biopsies of young patients obtained by CO₂ and Er:YAG laser surgery. Hematoxylin-eosin and Giemsa stains, PAS/diastase treatment, AE1 and AE2 cytokeratins, MiB1/Ki67, and bcl-2 immunoreactions were performed on the laser cut edges on formalin fixed, paraffin embedded biopsies. Results: CO₂ laser biopsies show blisters, clefts and erosions of the epithelium. Intracellular edema and lengthened nuclei were also seen. The glycogen content results decreased in CO₂ laser biopsies. Good expression for cytokeratins and cell-cycle proliferation markers were found in Er:YAG biopsies, on the contrary the apoptosis marker was better expressed in CO₂ laser biopsies. Conclusion: The results suggest that Er:YAG laser may be routinely used in surgery, because of its minimal damage of the epithelial tissue, its low inflammatory reaction, its quicker healing process and its lower risk of scarring.