Alteration of Skin Temperature during Low-Level Laser Irradiation at 830 nm in a Mouse Model

Abstract

Objective: This study investigated the change in local skin temperature in black and white mice during irradiation at 830 nm. Background Data: The photostimulation effect low-level laser therapy (LLLT) (700–900 nm) is widely accepted and used. However, the exact biological mechanisms of biostimulation are not yet established. Materials and Methods: Groups of C57BL/6J and BALB/cJ mice (n = 12 in each group) were lightly anesthetized with 50% carbon dioxide and 50% oxygen. The dorsum was shaved and a 1.0 × 0.5 cm spot was marked in the same location on each subject. Animals were photo-irradiated with a diode laser (CW, 830 nm, 36 mW output at 5 cm distance). Fluences of 0.0–5.0 J/cm² were delivered. Skin surface temperature was monitored by a thermal camera. Two thermocouples were placed 1 mm below the skin surface at the site of light exposure. Results: Temperature increased with increasing fluences of exposure. The surface temperature change at 5.0 J/cm² was 6.25 × 10^-2 ± 2.0 × 10^-3 vs. 1.2 × 10^-2 ± 3.0 × 10^-3 °C/mW for black and white mice, respectively. The temperature change at 1.0 mm depth was 4.51 × 10^-2 ± 3.0 × 10^-3 vs. 0.83 × 10^-2 ± 1.0 × 10^-3, respectively. Conclusion: CW irradiation at 830 nm and 5.0 J/cm² fluence induces a small temperature increase at the surface and at 1 mm in depth. The smaller effects seen in white mice might be due in part to reflection. This suggests that the thermal effects of irradiation at 830 nm are unlikely to explain the LLLT effect. However skin color should be considered, particularly at higher fluences. Further investigations are warranted to correlate the melanin content of the skin with observed LLLT effects.