Epitope Analysis of the MAb 1AD9 Antibody Detection Site in Human Protein Kinase CK2 α -Subunit

Abstract

1AD9 is a murine monoclonal antibody (MAb) that was produced against the recombinant human α-subunit of protein kinase CK2, a pleiotropic and constitutively active serine/threonine protein kinase. We have further characterized this antibody, which is suitable for Western blot, immunoprecipitation and enzyme-linked immunosorbant assay tests. Using an overlapping peptide library, we have identified the epitope targeted by MAb1AD9 characterized by the following sequence: ³¹⁹MEHPYF³²⁴.