Monoclonal Antibody against Free β -Subunit of Human Chorionic Gonadotropin

Abstract

Spleen cells from BALB/c mice immunized with free native human chorionic gonadotropin hormone β-subunit (βhCG) were fused with mouse myeloma cells (P3/X63-Ag8) and one hybridoma secreting monoclonal antibodies (MAbs), was obtained. This hybridoma specifically recognizes βhCG and does not cross-react with other human glycoprotein hormones, such as luteinizing hormone (LH), follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH), and human chorionic gonadotropin (hCG). The MAb was of the IgG₁ subclass and ascitic fluid from this hybridoma was purified by affinity chromatography on Protein A-Sepharose CL-4B column to isolate the IgG₁ active fraction. The affinity constant of this MAb was 1.5 × 10¹⁰M^-1.